CONTENTS №10-2015

3  Makarov V.V., Sukharev O.I., Gulyukin M.I. Diseases transmitted by Culicoides (orthobunia- and rhabdoviral infections)

This second part analytical paper deals with the main considerations on the etiology, pathology, nosogeography, parasito-systemic particularity, epidemiology for 8 the mammalian Culicoides-born infections, caused by viruses of genuses Orthobuniavirus of Buniaviridae family, Ephemerovirus and Vesiculovirus of Rhabdoviridae family. Key words: arboviruses, Culicoides, transmission.

PRACTIC: EXPERIMENT, PROBLEMS, PERSPECTIVES

8  Neustroev M.P., Tarabukina N.P., Zhirkov A.D., Tatarinova S.S., Pavlova A.I. Prevention of mycotoxicosis in horses by drug Sahabaktisubtil

Testing of probiotic Sahabaktisubtil show that the drug preventes mycotoxicoses, increases immunobiological reactivity and normalizes intestinal microbiocenosis in horses. Key words: bacterial strains, horse, mycotoxicosis, probiotic.

11  Grebenkin D.A. Natural milking is guaranteed profit

Natural milking is the process of milk getting by a calf from a cow. With the natural milking udder health of the cow always remains at a high level. Such a milking system ensures the best care for the nipple skin and its sphincter (at the expense of nipple massage quality) that provides a quick and painless milking, comfortable for cows. The purpose of machine milking is a simulation of the calf sucking process. This article will focus on a new way of milking with the milking machine AktivePuls, in which is pronounced the nipple massage and the imitation of natural milking. AktivePuls became a breakthrough in machine milking and was presented at the international exhibition EVROTIER 2014, where it immediately received a silver medal of the German farmer's Association DLG. Key words: cows, milking, milking machine, natural milking, nipple massage.

14  Lozovoy D.A., Starikov V.A., Mikhalishin D.V., Lyozova T.N. Immunobiological properties of sorbed vaccine type A, O, Asia-1 FMD for small ruminants

Results of studying immunobiological properties, safety of type A, O and Asia-1 FMD sorbed trivalent vaccine (from virus grown in BHK-21 cells) in small ruminants are presented in the paper. Provided indirect evidence of the absence in the vaccine non-structural proteins of the agent. Key words: foot and mouth disease virus, nonstructural proteins, vaccine, virus-neutralizing antibodies, reactogenicity, small ruminants.

17  Levitskaya I.L. Millennium – a guaranteed solution in case of animal clostridiosis

Clostridium is spread among all types of animals that is the reason of cost-effectiveness decrease of production. Using Millenium vaccine you have an opportunity to solve the problem of 11 types of clostridium of swine and cattle. Key words: clostridiosis, animals, vaccine, Millenium.

18  Boytsov A.V. Dosatron's medicators for industry livestock

Thearticle observes modern equipment for injection of medicines and feed additives to animals via drinking water. Key advantages and wide range of application of Dosatron medicators in animal husbandry are brought into limelight. Special attention is paid to dosators of the D25 series - the most popular in poultry industry and pig-breeding, as well as to the innovative DIA4RE model with the high resistance reinforced diaphragm (triple layer). Key words: dosator, medicator, Dosatron, dosing, vacination, desinfection, hygiene.

INFECTIOUS DISEASES

21  Yuzhakov A.G., Bogdanovа O.Yu., Grebennikova T.V., Hametova K.M., Musienko M.I., Verhovskij O.A., Aliper T.I., Zaberezhnyj A.D., Guljukin M.I. Experimental Schmallenberg virus infection of lambs and evaluation test system based on real-time PCR to detect viral RNA

Schmallenberg disease (SD) is a new viral infection of ruminants characterized by fever, diarrhea, abortion, birth of non-viable and/or malformed lambs and calves with joint malformations, scoliosis, torticollis and/or hydrocephaly. The infection is caused by an RNA virus (SBV) of Orthobunyavirus genus (Bunyaviridae family) and is related to the Simbu serogroup. The purpose of the present study was to re-create SD infection under experimental conditions using a viral strain isolated in Western Europe and to develop an RT-PCR assay to detect SBV RNA as well as to assess the distribution of SBV across the tissues and organs in infected lambs. The MU-2013 strain kindly provided by professor J.M. Sanchez-Vizcaino of Complutense University of Madrid (Spain) was used to infect the animals. Serum samples were analyzed with Ingezim Schmallenberg, a commercially available SBV antibody ELISA kit manufactured by Ingenasa (Spain). A reverse transcription real-time PCR (RT-qPCR) assay for detection of viral RNA in serum and organs of infected animals was developed. A plasmid containing a green fluorescent protein gene was used as an internal control alongside primers and the probe synthesided for its amplification. For reference purposes German-made primers and probe recommended by OIE we also used. Experimental infection of lambs with Schmallenberg disease resulted in a short period of viremia (2 - 4 days) and an almost complete absence of clinical signs. The virus was found to be circulating in the lymphatic system, particularly in the spleen, submandibular and premaxillary lymph nodes. Key words: Schmallenberg virus, real-time PCR, experimental infection.

26  Lenchenko E.M., Antonova A.N. Indication of virulence factors of Enterobacteria

There were tested and matched effective research methods differentiation invasive and cytotoxic strains of Enterobacteria producing adhesive antigens, bacteriocins, hemolysin, heat-lable & thermostable toxins. Key words: adhesive antigens, digestive diseases, laboratory models, pigs, heat-labile and heat-stable toxins, culture media, test-systems, chickens, Еnterobacteria.

INVASIVE DISEASES

31  Vasilevich F.I., Beginina A.M. Epizootologic monitoring of ixodes ticks in the Kaluga region

Monitoring show that Dermacentor reticulatus and Ixodes ricinus are the most widespread species of ixodes ticks in the Kaluga region. Ixodes persulcatus in this region was not found. Key words: anaplasmosis, ixodes ticks, tick borreliosis, parasites, natural-focal diseases.

32  Glazyev E.N., Arkhipov I.A., Balyshev A.V., Dragunkina O.S., Zubarev V.N. Efficacy of drug Ritril applied at helmithosis of small ruminants

 Anthelmintic drug Ritril including Ricobendazole and Triclabendazole demonstrated 98 – 100 % efficacy in treatment of monieziosis, dictyocaulosis, nematodiroses and strongylatosis of gastrointestinal tract. Trichocephala spp., Dicrocoelia spp. and Fasciola spp. were more resistant to the Ritril. Therefore it is recommended to use the drug at a higher dose 1,6 ml/10 kg of body weight. Sheep and goats well tolerated the drug without local and general side effects. Key words: antihelminthic, helminthiasis, goat, sheep.

36  Andreeva J.N. Monimax® is perfect coccidiostat

Experience the modern bird cross ROSS 308 convincingly proved that Monimax® is a unique synergistic combination ionophore coccidiostat monensin and chemical drug nicarbazin. The product provides an additional gain of body weight of the bird and improves the feed conversion ratio compared with the "classical" monotherapies and the combination of narasin/nicarbazin. It is highly effective against coccidia, fully preserving the intestinal health and growth energy of the body. It is completely safe and non-toxic at a dose of 75 ppm. Monomax® is produced in the form of microgranulate, which provides excellent flowability and mixability it with food. Active substance (monensin and nicarbazin) protected by a shell, they are gradually released throughout the intestine, influencing coccidia. Key words: coccidiosis, Monimax® Total Mean Lesion Score, conversion of a forage.

OBSTTETRICS, GYNECOLOGY

39  Korochkina E.A., Plemyashov K.V., Romanenko L.V., Uvarova A.S. Innovative approach to the mineral feeding of dairy cows in the transit period

Testing of new developed macro mineral boluses "Calcium Intensive" and "Calcium Intensive Plus” of national production (St. Petersburg) was made on dairy cows in transit period. It was found that administration of mineral feedings according to the scheme: the first bolus in 9-18 days before calving, the second bolus – in the calving day is an effective way of Calcium provision. Key words: dairy cows, metabolism of calcium and phosphorus, macro mineral boluses of national production, transit period.

42  Krutyakov Yu.A., Simonov P.G., Khaperskiy Yu.A., Violin B.V., Fedotov S.V. Effect of intrauterine nanosilver on reproductive performance of lactating dairy cows diagnosed with chronic endometritis

The objectives of the study were to assess effectiveness of a new antibacterial nanosilver-based veterinary drug Argumistin® (10 ppm of silver nanoparticles, 100 ppm of benzyldimethyl[3-(myristoylamino)propyl]ammonium chloride monohydrate) in treatment of cows with chronic endometritis when administered intrauterine. One experimental and one control group in total included 56 Holstein cows (average milk yield per cow per year 6500 – 7000 liters) with chronic endometritis from Komsomolskaya Dairy Farm (Altai region, Russia). The reference drug used in the control group contained benzethonium chloride as active component. The use of Argumistin® resulted in significant improvement of the reproductive performance of cows and their recovery from chronic endometritis. Key words: chronic endometritis, colloidal silver, miramistin, Argumistin®, infertility, nonspecific resistance.

46 Masyanov Yu.N., Manuylov A.V., Kotsarev V.N., Brigadirov Yu.N., Mikhaylov E.V., Filippova M.V. The method of obtaining cervical-uterine mucus in sows for conducting immunobiochemical researches

The method of obtaining cervical-uterine mucus in sows was developed with the aid of a set, allowing to get it in sufficient amount for conducting complex immunobiochemical researches with the purpose to diagnose latent inflammatory processes in reproductive organs. Key words: sow, latentendometritis, cervical-uterine mucus, device.

ABROAD

49  Mukminov M.N., Nikitin O.V., Ndayishimiye E.W., Shuralev E.A. Assessing the prospects for beekeeping development in Southern Province of Zambia

There are present the results of study the content of heavy metals in the samples of termite mounds and soils in the Southern Province of Zambia. The parameters were higher than maximal permissible burden in Livingstone and Siavonga regions. On this base the conclusion was made that it is inexpedient to placement apiaries on these territories. Key words: accumulation, bioindicator, biogenic component, bee, termite mounds.

LABORATORY PRACTICE

53  Morgounov Yu.P., Malogolovkin A.S., Morgunov S.Yu., Burmakina G.S., Kushnir S.D., Yurkov S.G., Tsybanov S.J., Kolbasov D.V. Biological characteristics of COS-1 cell line adapted African swine fever virusstains of 8 seroimmunotype

The article presents the experimental data concerned adaptation of African swine fever virus 8 seroimmunotypeisolated in Russia in 2008 – 2012 to COS-1 cell line. The COS-1 adapted ASFV strains Tver/12c and Volgograd/14c were obtained. Here we present also the biological characteristics of ASFV adapted strains and the possibility of using them for virological and molecular studies. Key words: African swine fever, adaptation, permanent cell line, the response delay hemadsorbtion, typing, culture of bone marrow cells.

58 Satyukova L.P. Determination of dioxins and dioxin-like substances by method Dr. Calux® in eggs and tissues of broilers

Excess of residual amounts of dioxins and dioxin-like substances often become a cause of mass poisoning of animals and people, discarding of livestock products. Practical issues in the study and proposal of new methods for the detection of dioxins and related compounds in various biological objects is extremely important. Key words: dioxins and dioxin-like substances, methods of determining screening method Dr. Calux®, the impact of dioxin on the body agricultural poultry.

61        FROM VETERINARY MEDICINE HISTORY

 

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CONTENTS №10-2015