CONTENTS №3-2015

3          Smirnov A.M. The main stages in the development of All-Russian Research Institute of Veterinary Sanitation, Hygiene and  Ecology – FANO

This year we celebrate the 80th anniversary of the creation of VNIIVSGE. Throughout such long period the Institute saved status of the head research organization in the field of veterinary sanitation, hygiene and ecology. Studies carried out by its scientists provide veterinary welfare of livestock, production quality and sanitary safe products, raw materials of animal origin and feed, protection the environment from pollution from livestock farms. Key words: animal husbandry, ecology, environment, hygiene, sanitation, veterinary.

PRACTIC: EXPERIMENT, PROBLEMS, PERSPECTIVES

8          Khlopitsky V.P.,  Sidorchuk A.A., Shumskij N.I. Complex  control of infectious factors in the system of reproduction at livestock of pigs

In article the review of the basic infectious factors reducing reproductive indicators uterus a livestock of pigs is given. The complex of laboratory-diagnostic works for the purpose of increase of efficiency of veterinary actions in system of reproduction of pigs is offered manufacture. Key words: sows, male pigs, reproduction of pigs, porcine reproductive and respiratory syndrome (PRRS), porcine parvovirus infection, brucellosis suum, chlamydiosis, leptospirosis suum, «Polygene», «Amoximag®», «Acilomag®», « Enromag®».

12        Aliyev A.S., Gromov I.N., Beginin G.V., Petrunin S.A. The efficacy of vector-vaccine and inactivated vaccine by specific prevent of infectious bursal disease

The article discusses the results of production tests on the comparative evaluation of the effectiveness of the vector and inactivated vaccines against infectious bursal disease for specific prevention of the infection. Key words: infectious bursal disease, vector-vaccine, inactivated vaccine, seromonitoring, antibodies, ELISA.

INFECTIOUS  DISEASES

17        Arakelyan P.K., Dimov S.K., Dimova A.S., Arapchor M.Sh-O., Kerimova G.O., Baulin E.M., Zajkova T.A. The efficiency conjunctiva immunization with live vaccine from  strain B. abortus 19 small ruminants

There was proved antiepizootic, anti-epidemic and prophylactic efficacy of conjunctival immunization of sheep and goats against brucellosis live vaccine from strain of B. abortus 19 at a dose of 4 billion microbial cells (reduced by 10 times compared with subcutaneous) in the whole complex antibrucellar activities at the disadvantaged and endangered areas. The conjunctival method of application of vaccine strain 19 has a harmless and easy to use. Its annual use in disadvantaged and endangered flocks allows unimpeded (thought 4 months after each immunization/reimmunization) to post-vaccination study animals in order to timely detect hidden carrier of brucellosis, control epizootic welfare of flocks, and its differential diagnostic evaluation using O-PS antigens from B.abortus and B.melitensis on brucellosis, caused B.melitensis. Key words: brucellosis , B. melitensis, small cattle , disadvantaged and threatened flock, immunization, live vaccine from strain of B. abortus 19, conjunctival method, postvaccinal diagnosis, differential epizootic evaluation, efficiency.

21        Starikov V.A., Mikhalishin D.V., Lyozova T.N., Mikhalishin V.V. Comparative examination of immunogenicity of sorbate and emulsion vaccines for cattle

Type A and O sorbat vaccines containing 6 µg of immunogenic components demonstrated insufficient activity for young animals and cattle. Following vaccination at early stages a universal sorbat vaccine induced virus neutralizing antibodies at a rate of 6.40-7.40 log2 but the titre decreased up to 5.00±0.27 log2 by day 90. Emulsion vaccines created the same level of humoral immunity in cattle by day 14-21 after vaccination as a universal sorbat vaccine. The antibody titre did not change during the observation period (90 days). Key words: adjuvant, FMD vaccine, antibodies, antigen, FMDV, strain, immunifacient component, immunization, infection, cattle.

26        SpiridonovA.N.,Irza V.N., KonstantinovA.V.,   DolgovD.L., ZuevY.V.  The study of resistance to velogenic Newcastle disease virus in vaccinated pigeons

The main purpose of this work is to study resistance in vaccinated pigeons to highly virulent strain of Newcastle disease virus when using different vaccination schemes. Magnitude of immune response in vaccinated birds was assessed by hemagglutination inhibition (HI) assay. Experimental inactivated vaccine against ND induced high titer antibody response in pigeons and did not cause postvaccinal reactions of general and local character in pigeons. When challenged with highly virulent ND virus the pigeons immunized only with live or inactivated vaccine or with both vaccines at the same time developed no clinical signs compared to the birds in the control group where clinical signs related to ND and deaths were observed. Keywords: vaccine, pigeons, Newcastle disease.

INVASIVE DISEASES

30        NovikovaS.V., ZubarevV.N., DragunkinaO.S.Efficacy of the drug Ricazol against nematodes and trematodes of sheep

The new drug Ricazole with the active substance ricobendazole showed high anthelmintic effect against different types of nematodes and trematodes of sheep at a dose of 1 ml/12,5 kg body weight. Key words: sheep, albendazole, antihelminthic, ricobendazole.

OBSTTETRICS, GYNECOLOGY

32        PlemyashovK.V., DmitrievaT.O., PotapovaA.Yu. Synthetic Beta-carotene in the stabilization of the estruse cycle in dogs at avarian disunction

The article describes the causes of ovarian dysfunction and disorders of estrous cycle in dogs. A method of the prevention and correction measures based on synthetic beta-carotene. In the first series of experiment 15 dogs over 5 years were studied, with an early manifestation of a estrus disorders. Ovaries were examined histologically after theovariogistereetomia. It was revealed cystic degeneration of follicles in 74,0 % of cases. In a second series of experiments the clinical efficacy of beta-carotenewas investigated. The use of the drug in breeding females (n = 90) during puberty worked as a prophylactic measures. It was justified from the point of view of prevention and treatment of ovarian dysfunction. Key words: estrous cycle, dogs, ovarian dysfunction, synthetically Beta-carotene.

ZOOHYGIENA, SANITATION, ECOLOGY

 35        SmirnovA.M., ButkoM.P., MkrtumianA.V., KudriavtsevE.A.,KorgevenkoG.N., TurinV.G. Mechanization of veterinary-sanitary events (state, problems and prospects)

The present state, problems and prospects of development of mechanization of animal health work, the prospects for development. Key words: mechanization, sanitary processing, systems of cars.

40        Prokopenko A.A., Zakomyrdin A.A., Bochenin Yu.I., Vanner N.E., Gruznov D.V. Directed aerosols of electroactivated solution to use disinfection in the poultry houses in case of colibacteriosis and aspergillosis of poultry

The regimes and technology of using the directed, aerosols of ANK neutral anolite were developed to conduct the disinfection in the poultry houses in case of colibacteriosis and aspergillosis securing irradiation of aerogenic infection agents in air circumstances as well as on the surfaces in houses and prevention of the infectious diseases. Key words: ANK neutral anolite, colibacteriosis, aspergillosis, installations, disinfection, house, regimes, technology, using.

44           BiryukovK.N. Survival test organisms in organic waste livestock at an accelerated composting

Set the duration of survival indicator of sanitary- exponential and pathogenic organisms in the muck with his active composting biofermentёre and defined modes of its disinfection. Key words: disinfection, organic waste, composting, pathogenic microflora, biofermenter.

 NONINFECTIOUS DISEASES

47        Engashev S.V., Dgalilov R.Yu.,  Novak M.D., Abramov V.E. Ketonorm – effective in preventing ketosis cattle

The results show a positive effect of the Ketonorm for patients with ketosis cows. Adaptogenic drug Ketonorm promotes energy potential and to stimulate the metabolic processes. Complex preventive measures in dairy complexes with a high risk state of acidosis, including the use of energy resources as Ketonorm will help preserve the health and optimize the service life of dairy cattle to increase milk production, reduce the likelihood of intrauterine pathology and increase the susceptibility of calves in the early postnatal period to infectious diseases. Key words: cattle, ketosis, Ketonorm, biochemical parameters.

50        MedvedevI.N., ParahnevichA.V.Krezacin and Gamavit for correction of the curtailing system of blood at newborn pigs with the most widespread violations

The newborn piglets with acute hypoxia coagulation activation develops, which creates the risk of thrombosis in vessels. Use Krezacin and Gamavit for these animals is able to fully normalize coagulation activity of plasma. Key words: deficiency of iron, acute hypoxia, piglets, the newborn phase, Krezacin, Gamavit, out of the blood.

PHARMACOLOGY AND TOXICOLOGY

54        BoudarkovV.A.Сomparative evaluation of economical efficiency of keeping hens subjected to a chronical effect of 131I

Comparatively low doses of 131I (namely, 0,11 and 1,1 MBq/kg) did not change drastically or raised the productivity levels while some higher doses (>2,1 MBq/kg) resulted in a severe decrease in the product output. These changes were predominantly caused by malfunctions of poultry egg-laying qualities. The offspring of these hens kept up to 3-month age demonstrated some drop in their productivity levels. The productivity decrease rates as observed for the offspring correlated with the dosage of the radionuclide having been delivered into the organisms of their parents, so that the higher the dose was used, the lower the product output level was noted. Кey words: 131I, hens and their progeny, economical efficiency of keeping.

 LABORATORY PRACTICE

57        Kotova О.Ju.,  Suvorova Ju.А.,  Кushnir S.D.,  Jurkov S.G., Balashova E.A.  Cytomorphological changes in african green monkey kidney (CV-1) continuous cell line induced with Akabane disease virus

This paper presents the results of Akabane disease virus Strain V8935 replicates in a continuous cell culture CV-1 without any prior adaptation. And with  cytopathic effect manifestation. Key words: virus, Akabane disease, cell culture, CV-1, cytopathic effect.

60        Khanyukova E.U., Shustova M.A., Pyatkina A.A. Dependance of turkey herpesvirus reproduction in chicken embryo fibroblast (cef) cell cultures on medium

In the paper the cultivation of SPF-chicken fibroblast cells inoculated with turkey herpesvirus using different commercial nutrient media is studied. It has been established that Dulbekko DMEM/F-12 Ham nutrient provides the highest yield of virus-containing cells as well as the highest infectivity of the material. Key words: turkey herpesvirus, medium, SPF-chicken embryo fibroblasts.

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CONTENTS №3-2015