CONTENTS №4-2025

3 Makarov V.V., Stekolnikov A.A., Sochnev V.V.  Factor-endogenous doctrine of the main pathology of productive animals (part 2)

The second part of this report the epizootology of factorial and opportunistic infections of animals, the psychoneuroimmunology as a susceptibility factor, microbiology and nosology are considered. Key words: epizootology, “doctrine” of the mechanism of infection transmission, factorial and opportunistic infections.

DOI:10.30896/0042-4846.2025.28.4.03-07

EXPERIMENT, PROBLEMS, PERSPECTIVES

9 Rykova V.S. Wild boars as a natural reservoir of viral infections of swine

Wild boars are often considered as a potential reservoir of porcine viral infections, including African swine fever, classical swine fever, Aujeska disease and porcine reproductive and respiratory syndrome. The current paper delineates up-to-date data regarding this problem. Key words: wild boars; swine viral diseases; pig farming.

DOI:10.30896/0042-4846.2025.28.4.09-14

INFECTIOUS  DISEASES

16 Anoyatbekova A.M., Prokhorova P.V., Kucheruk O.D., Gulyukin A.M. Molecular genetic characteristics of Pestivirus scrofae identified in pigs in a farm of Belgorod region

The results of the study of 223 samples of clinical and pathological material from pigs obtained from a pig farm in the Belgorod region tested for the presence of Pestivirus scrofae (Atypical porcine pestivirus – APPV), and its molecular genetic characteristics are presented in this article. The overall prevalence of APPV among pigs of all tested groups was 24,6 %. Phylogenetic analysis demonstrated that the Belgorod-11/25 isolate has a high nucleotide similarity with isolates from Hungary (99,3 %), Germany (94,6 %), and the USA (93,4 %) and belongs to genotype I subgenotype 3 (1.3) of APPV. Key words: Atypical porcine pestivirus, Pestivirus scrofae, PCR, genome, phylogenetic analysis.

DOI:10.30896/0042-4846.2025.28.4.16-21

22 Filatov I.E., Korneeva Yu.V.,  Tsibezov V.V., Alekseev K.P., Yuzhakov A.G., Aliper T.I., Verkhovsky O.A. Enzyme-linked immunoassay of IgG antibodies to rotavirus type A in pigs' blood serum

The article focuses on developing a new test system based on indirect enzyme-linked immunosorbent assay to detect IgG antibodies to rotavirus type A in pig blood serum. Recombinant proteins VP2 and VP6 rotavirus type A were obtained, and conditions for their production and purification were worked out. High specificity and sensitivity of the test system were established. By the means of developed test system, sera of pigs from industrial pig complexes of the Russian Federation were analyzed, and the patterns of RVA-specific IgG antibody content in animals of different age and sex groups were established. Key words: rotavirus type A, rotavirus gastroenteritis, pigs, ELISA.

DOI:10.30896/0042-4846.2025.28.4.22-28

OBSTTERICS, GYNECOLOGY

29 Kolyadina N.I., Obukhov I.L., Khusnetdinova N.F. Ultrasound evaluation of follicles and oocytes of cumulus complexes in planning ART in dogs

The relationship between the ultrasonographic picture of follicular dynamics, ovulation and early postovulatory period with the level of progesterone and visualization of the oocyte-cumulus complex (OCC) in the follicular cavity was revealed. An algorithm for monitoring females during assisted reproductive technologies (ART) was developed, allowing to increase the percentage of successful fertilization. Key words: assisted reproductive technologies, females, follicles, oocyte, oocyte-cumulus complex, ovulation, fertilization, ultrasound.

DOI:10.30896/0042-4846.2025.28.4.29-33

PHARMACOLOGY AND TOXICOLOGY

35 Vertiprakhov V.G., Galyga S.D., Lopatina V.V. Effect of trypsin injections on hemodynamics and blood biochemical parameters in rabbits after ovarioectomy

In rabbits after ovarioectomy there is an increase in blood pressure by 14.6% compared to intact animals. Analysis of individual features of changes in hemodynamic parameters in rabbits showed that after intramuscular injection of trypsin blood pressure normalizes: at baseline low level - increases to normal, and at baseline high, on the contrary, decreases. Biochemical indices of blood indicate a decrease in the rate of protein metabolism, as trypsin activity in the blood of experimental rabbits decreases by 40,9 %. The content of triglycerides in the blood decreases, which is due to lower lipid assimilation in the intestine or efficient processing in the liver. There is a decrease in blood calcium content and an increase in phosphorus, which is apparently due to the increased catabolic processes in the body of rabbits after ovarioectomy. Key words: trypsin, blood pressure, blood biochemistry, ovarioectomy, rabbits.

DOI:10.30896/0042-4846.2025.28.4.35-39

39 Kashevarov G.S., Tarasova E.Yu., Matrosova L.E. Study of the cytomorphology of broiler chickens myocardium and spleen in mycotoxicosis against the background of the use of Galluasorb

The article presents the results of a study on changes in the myocardium and spleen of broiler chickens at the ultrastructural level when exposed to mycotoxins. Morphological changes at the ultrastructural level of both white pulp cells of the spleen and cardiomyocytes of broiler chickens of the toxic control group were revealed. Ultrastructural changes when using «Galluasorb» as an adsorbent were less pronounced and occurred less frequently, which indicates its protective effect. Keywords: combined mycotoxicosis, complex adsorbent, myocardium, spleen, ultrastructure, broiler chickens.

DOI:10.30896/0042-4846.2025.28.4.39-43

44 Marinicheva M.P., Strogov V.V., Kozlov S.V., Fokin A.I. Effect of iodine aerosol application on the broiler chickens

Raising broiler chickens without the use of antibacterial drugs is an urgent task of modern poultry farming. Broilers grow fast, but they have enough resistance to pathogens. Therefore, poultry farmers face a very difficult task – to find effective and safe medicines for poultry and poultry products. There is such an antibacterial drug based on iodine on the domestic market – Dixam Extra in powder form. The authors used it aerosolized in the cultivation of ROSS 308 cross broilers and analyzed the effect of the drug on the body of chickens. The results showed that the drug Dixam Extra in the form of an inhalation aerosol at a dose of 5 mg of iodine per 1 m3 and an exposure of 30 minutes did not cause any abnormalities in the morphological composition of the blood, on the contrary, hematopoiesis proceeded more intensively. The drug had no negative effect on the general condition of the bird and increased the dynamics of average daily weight gain. Key words: blood, average daily weight gain, iodine, inhalation effect, poultry, chickens, broilers, ROSS 308.

DOI:10.30896/0042-4846.2025.28.4.44-47

48 Galimova V.P., Blinov N.V. The effect of the enterosorbent Polysorb MP on the dynamics of residual amounts of clotrimazole in honey

The article is devoted to the study of the possibility of using the enterosorbent Polysorb MP for the binding of clotrimazole in honey. Laboratory studies have shown that the enterosorbent introduced into contaminated honey reduces the duration of the precense of clotrimazole and reduces the amount of its residues. Key words: honey, clotrimazole, residual amounts, enterosorbent Polysorb MP, HPLC.

DOI:10.30896/0042-4846.2025.28.4.48-50

FROM VETERINARY MEDICINE HISTORY

52 Smirnov A.M., Popov P.A., Gunenkova N.K. All-Russian Research Institute of Veterinary Sanitation, Hygiene and Ecology: main stages of development and achievements (the 90th anniversary of its foundation)

The article presents the main stages of development and directions of scientific activity of the All-Russian Research Institute of Veterinary Sanitation, Hygiene and Ecology - branch of the Federal Scientific Center – All-Russian Research Institute of Experimental Veterinary Medicine named after K.I. Skryabin and Ya. R. Kovalenko of the Russian Academy of Sciences in connection with the 90th anniversary of its foundation. The institute is at the head of veterinary and sanitary science, being the only veterinary research institute on sanitary issues in animal husbandry and veterinary science. The scientists of the institute conduct theoretical and applied research aimed at ensuring the veterinary well-being of animal husbandry, obtaining high-quality and sanitary-safe products, raw materials of animal origin and feed, protecting animals from natural and anthropogenic pollutants and protecting the environment from pollution associated with the activities of livestock complexes and the use of chemical means of animal protection. Key words: objects of veterinary supervision, disinfection, neutralization, environmental assessment.

DOI:10.30896/0042-4846.2025.28.4.52-55