CONTENTS №5-2015

3       Shabunin S.V., Shakhov A.G., Chernitskiy A.E., Zolotarev A.I., Retsky M.I. Respiratory diseases of calves: a modern approach to the problem

Respiratory diseases of calves are one of the most acute problems of dairy and beef cattle breeding in conditions of their intensification. The paper discusses the etiology and pathogenesis of respiratory diseases, their relation with the level of dams’ health, formation of respiratory, immune systems and metabolism in neonates, gastro-intestinal diseases and omphalitis. It is the first time the methods of prediction of respiratory diseases development in newborn calves are presented.  Much attention is paid to issues of clinical and laboratory diagnostics. The authors give recommendations for the prevention and control of respiratory infections in calves and expound the main principles of treatment of sick animals. Key words: calves, respiratory diseases, spread, etiology, pathogenesis, prognosis, diagnosis, treatment, prevention, epidemiology, control measures.

PRACTIC: EXPERIMENT, PROBLEMS, PERSPECTIVES

14        Kalinin A.N., Rozhdestvenskaya T.N. , Krokhin N.L. Etiology of the respiratory syndrome of birds in the industrial poultry farming

The survey provides information on the etiology, predisposing factors, diagnosis and control of respiratory syndrome birds. Key words: environment, etiology, industrial poultry farming, respiratory syndrome, specific prophylaxis.

17  Grigorev A.V., Novikova S.V., Abramov V.E.,  Glukhareva E.V.,  Balyshev A.V.,  Osyanina M.N. Improving etiotropic therapy of acute bronchitis in calves

There are present data on the effectiveness of a new injectable drug for the treatment of Azitronit acute bronchitis  in calves compared with different groups of antibiotics. Key words: antibiotic therapy, azithromycin, calves, respiratory diseases, therapeutic efficacy.

 INFECTIOUS  DISEASES

20       Aliev A.S.,  Burlakov M.V.,  Gromov I.N.,  Selikhanova M.K., Zhurov  D.O.,  Emelyanova S.A. Pathogenicity of isolates of сhicken infectious anemia virus

The results of study the biological properties of 3 isolates chickens infectious anemia virus isolated in Russia are reptresents. Key words: broilers, chicken infectious anemia virus, pathogenicity.

25        Volkov S.A., Bessolitsyna E.A., Darmov I.V., Stolbova F.S. The research of ticks infestation with Anaplasma rickettsia on a territory of Kirov region

The problem of transmissible diseases is becoming widely spread nowadays. Anaplasmosis is one of such diseases. The aim of this research is to show the percentage of ticks contamination depending on species of ticks, their gender and object from which it were gathered with the help of PCR. Also it's necessary to develop conditions of PCR. The object of this study are ticks of Dermacentor and Ixodes genus which were gathered in different areas of Kirov region. Thicks were gathered from animals, people and plants. During the research 283 samples of thick were studied. It was shown that the average percentage of contamination is about 22 – 28%. Also it war proved that carrying agents can be not only female, but male too. The major vector is Dermacentor reticulatus but Ixodes persulcatus can also serve as a vector in Kirov region. Key words:  Ixodic  ticks, Anaplasma, DNA, PCR,  identification.

INVASIVE DISEASES

30      Bittirov A.M., Toкhaeva A.I., Midova L.A., Bittirova A.A. Species composition helminthes sheep of the Stavropol and north Caucasian rocks in the North Caucasus

Specific composition helminthes sheep Stavropol and North Caucasus rocks in homestead farms prairie zone region North Caucasus presented accordingly, 36 and 24 views. In sheep, both species are significant epizootological nematode genera Ostertagia; Nematodirus; Bunostomum; Oesophagostomum; Trichostrongylus; Chabertia; Fasciola; Dicrocoelium; Echinococcus; Haemonchus and Trichocephalis. On average, 5,7 per sheep defined types of helminthes, that should consider at conduct forced and preventive deworming. Key words: North Caucasus, helminthes, view, fauna, sheep, breed, Stavropol, a North Caucasus, extensive and intensive invasions.

32        Bundina L.A., Engashev S.V.  Prevalence of intestinal nematodes and effectiveness of horse deworming in sport clubs of Moscow region 

There are presents data on the spread intestinal nematodes among sport horses in the Moscow region, as well as the effectiveness of use against them different anthelmintic. Key words: alben, anthelmintics, helminthes, horse, pony, pasta Alezan, febtal, Parascaris equorum, Strongilata sp.

OBSTTETRICS, GYNECOLOGY

36        Degtarev V.P., Fedotov S.V., Udalov G.M. Symptomatic infertility in cows caused genital infections

On the basis of data analysis of livestock dairy cows Kaluga oblast and Altai Krai established a clear relationship between reproductive statuses of the cows with the presence of sexually transmitted infections. Key words: reproductive status, vaginal form of infectious bovine rhinotracheitis, mycoplasma, endometritis, reproductive activity of the herd.

39        Narizhnyi A.G., Dzhamaldinov A.Ch., Kreindlina N.I., Anisimov А.G., Fainov A.A. Reproductive Performance in Boars, Using Hepatoprotective Preparations

The influence of the introduction of the basic diet of breeding boars of biologically active substances contained in the seeds of milk thistle and essential phospholipids on the functional state of the body and reproduction rates. It was found that the highest rates obtained by the integrated use of these drugs. Keywords: essential phospholipids, drugs thistle, breeding boars, blood counts, sperm, fertility of sows.

NONINFECTIOUS DISEASES

43        D'iachkov A.N., Emanov A.A. X-ray dynamics filling acute posttraumatic defects of canine leg bones by the transosseous osteosynthesis method according to Ilizarov

The experiment performed in 24 adult mongrel dogs by modeling an open comminuted leg fracture in them. After that, primary debridement made in the operating room with removal of bone fragments (defect amounted to 24,9±0,7 % of leg length), transosseous osteosynthesis performed with the Ilizarov fixator. Distraction started on Day 7 after the surgery, and it lasted for 72 – 106 days. By 14 days of distraction the zonation of regenerated bone determined, by the middle of distraction – additional translucent zones, and by the end of lengthening the growth plate crossed all over with longitudinally oriented shadows. The period of fixation was 92,7±1,6 days on the average. Organotypical reorganization of the newly formed bone part occurred in a year after the fixator removal. Key words: fracture, bone defect, experiment, dog, leg, the Ilizarov fixator.

47        Burkov P.V., Shcherbakov P.N. A new approach to the regeneration of liver cells by means of the modified cytotoxins

There were studied the effect of the drug Geprime for chicken on the formation of binucleated hepatocytes, which employes marker of regeneration liver cells. It was established that cytotoxins of this drug stimulate increase the number of such cellular elements. Key words: hepatocytes, liver, regeneration, cytotoxins.

PHARMACOLOGY AND TOXICOLOGY

49        Nikolaenko V.P., Mihaylova A.V. Aerosol appliance of the new remedy Laktosept for broilers

The studies present the results of aerosol Laktosept appliance research in broiler growing. The positive effect of Laktosept on resistance of chickens, the increasing of vitamins A, E, B2, body weight gain and mortality was stated. Key words: broilers, Laktosept, body weight, livability, resistance, vitamins.

52        Gavrikov A.V., Chukina S.I., Koveshnikova E.I., Avchuk S.I., Dolgova G.V., Novik T.S. The study of acute toxicity Amoksimag

In experiments on laboratory animals was studied acute toxicity of Amoximag®, 15% injectable amoxicillin suspension. Key words: amoxicillin, toxicity, mice, oral, intramuscular.

VETERINARY-SANITARY CONTROL

55        Kochish I.I., Smirnov S.L., Gerasimov M.A., Semikrasova A.N. Influence of Viroсide on the quality slaughter products of rabbits

Two-time aerosol disinfection of premises by Virocide solutions of different concentrations did not effect on the quality meat and slaughter products of rabbits kept in them. Key words: aerosol disinfection, meat, rabbit, slaughter products, veterinary-sanitary examination, Virocide.

LABORATORY PRACTICE

58           Tsibezov V.V., Terekhova Yu.O., Verkhovsky O.A., Pershin А.S., Vlasova N.N., Igolkin A.S., Chernykh O.Yu., Aliper T.I., Nepoklonov E.A. Efficacy of the immunochromatographic method for the rapid detection of African swine fever virus

An immunochromatographic one-step assay was designed for rapid detection of the African swine fever virus (ASFV). The assay is based on two different monoclonal antibodies that bind to distinct epitopes of the viral protein vp72, one of which was labeled with colloidal gold and coated onto a predetermined site on a glass fiber membrane, whilst another antibody was immobilized on the surface of a nitrocellulose membrane. Diagnostic sensitivity and specificity of this immunochromatographic assay were compared to those of commercial ELISA and PCR kits for samples collected from experimentally challenged and spontaneously infected domestic pigs and wild boars. A 100% sensitivity and specificity were demonstrated for the assay as assessed using blood and spleen samples from sick and fallen animals with a high level of viremia. It was concluded that the assay is suitable for the detection of the African swine fever virus both in laboratories and in the field. Key words: African swine fever virus, immunochromatographic assay, PCR, ELISA, IFA.

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CONTENTS №5-2015