CONTENTS №11-2017
3 ZhuravlyovaV.A., LyskaV.M., VasilievA.P., ShkaevA.E., StrizhakovaO.M., SidlikM.V. Structure of modern area of distribution African swine fever in the Russian Federation
There are presented the dynamics and structure of the modern distribution range of African swine fever in the Russian Federation. This information can be useful for planning measures of control ASF. Key words: African swine fever, epizootic process.
PRACTIC: EXPERIMENT, PROBLEMS, PERSPECTIVES
7 Aliev A.S., Gromov I.N., Musorina A.S., Gadzhiguseeva N.F. Isolation and characterization of the infectious anemia virus of chickens
The article presents the results of studies on the isolation and cultivation of isolates of the virus of infectious anemia in broiler chickens with signs of anemia in the cell culture of MDCC-MSV1, the study of their antigenic and pathogenic properties, as well as pathomorphological changes in diurnal SPF chicks experimentally infected Three isolates of this pathogen. Key words: infectious anemia virus, MDCC-MSV1 culture, cytopathic action, apoptosis, antigenic activity, pathogenicity, SPF chickens.
15 OkolelovaT.M., Yengashev S.V., SalgereevS.M. Cellular fatigue of laying hens: causes and prevention.
With the use of highly productive birds, specialists often face the syndrome of cell fatigue in laying hens, which cause significant economic damage. The authors describe the causes and give recommendations on the prevention of disease in birds of different ages. Key words: chickens, cellular fatigue, osteoporosis, causes, prevention.
19 Simavonyan K.V., Leontyuk A.V., Kaplanyan P.A., Chugueva I.I., Chuguev Y.P. Breast cancer in a female chlorocebus
A 17-year-old female chlorocebus died from breast cancer with metastases to the mesenteric lymph nodes, retroperitoneal space and the spine. Metastases in the spine caused paresis of the hind limbs. The clinic of the disease was complicated by diarrhea and osteomyelitis of the ischium. The results of clinical, laboratory, radiographic, sectional and histological studies are presented in the report. Key words: breast cancer, chlorocebus, mastitis, metastases, osteomyelitis.
INFECTION DISEASE
23 Yurov K.P., Alexeyenkova S.V., Pchelnikov A.V., Simanova I.N. Ethiological structure of acute bovine herpesviral infection
In 2011 – 2016 years in several commercial and large-scale farms in the period of outbreaks of acute respiratory infection from calves, viral strains were isolated and identified as bovine alphaherpesvirus type 5 by phylogenetic analysis. It is assumed that due to close similarity of antigens and genome structure, this virus can in some cases be mistakenly identified as the causative agent of the infectious bovine rhinotracheitis. A phylogenetic analysis of the virus isolates indicates that they are close to the "non-a-non-b" subtype of bovine herpesvirus of type 5. Key words: bovine alphaherpesviruses, infectious bovine rhinotracheitis, bovine herpesvirus type 5, nucleotide sequencing, PCR, phylogenetic analysis.
28 Poryvaeva A.P., Pechura E.V., Nurmiyeva V.R. Rhinocytogram in calves with infectious respiratory diseases
The percentage of neutrophils, lymphocytes, eosinophils and epithelial cells was determined in smears-prints from the mucous membrane of the nasal cavity of 35 calves with diseases of the upper respiratory tract. It was found that in the nasal mucosa of 14 clinically healthy calves flat epithelial cells predominated (up to 48,16±1,31 %), the number of leukocyte cells was 20 – 22 % and goblet epithelial cells – 12,6±0,94 %. 11 calves with PG-3 infection had an increase relative number of lymphocytes up to 19,4±1,2 %, cells of the goblet epithelium – up to 24,3±1,7 % (p≤0,05). These observations and the results of clinical and laboratory studies confirmed the inadequacy of a complex of specific and nonspecific factors of nasal mucosa protection in calves infected by VPI-3. 10 calves with bacterial infection had in the rhinocytogram in addition to an increase in the number of neutrophils up to 29,2±1,9 %, a significant increase in the number of eosinophils up to 12,7±1,3 % (p≤0,05). A change in the composition of epithelial cells was also observed in these animals: the number of squamous epithelial cells decreased 2,4 times, goblet cells in 1,4 times. The revealed results local disturbances of the mucosa and in the submucosal layer of the nasal passages in calves with infectious respiratory diseases. Key words: infection, lymphocytes, neutrophils, parainfluenza, respiratory diseases, rhinocytogram, calves, cytomorphology, eosinophils, epithelial cells.
33 Boudarkov V.A., Kneize A.V., Shkayev A.E., Shkayeva N.A., Sadov K.M., Koustickova O.V., Bespalova T.Yu., Deuldeina T.V. Efficacy of software programs used for monitoring bovine virus leucosis in various geographical and environmental conditions
The report review application of some software programs for monitoring of bovine virus leucosis spreading in Chelyabinsk, Orenburg and Samara regions of Russia. The effective use of electronic databases in the development of a methodology for the improvement of cattle herds from this viral infection in various geographical and environmental conditions. Key words: bovine leucosis, epizootic process, software programs, monitoring.
INVASIVE DISEASES
39 Dashinimaev B.Ts., Boiarova L.I. Species composition of horse's digestive tract parasites in Zabaikalsky region
The results of study the distribution and species composition of mixed infestations digestive tract of horses in the Zabaikalsky region are received. The present study has showed that gadfly and helminth infections of the digestive tract of horses in this region are widespread. These parasites affect animals with extent of infestation 90,0 and 90,7 % respectively. Helminthes of the gastrointestinal tract of horses are represented by 43 species (42 nematodes and 1 cestoda). 18 species of helminths were identified for the first time in the Zabaikalsky region. It was observed that of the most numerous species of collected nematodes were from family Trichonematidae (93,56 %). All examined young animals up to 2 years were infested with helminthes. Their intensity of infection was up 512153 samples. Although all adult horses were infested, they had lower intensity of the infestation than young horses – 84480 samples. The maximum extents of infestation were detected in horses, which inhabit in forest steppe zone. Gastric gadfly have 5 species of which Gastrophilus intestinalis (large gastric gadfly) is most numerous (48,2 %). Key words: cestodes,extensiveness of invasion, helminthes, gastric botfly, horse, intensity of invasion, nematodes.
44 Nikanorova A.M., Vasilevich F.I. Dirofilaria of dogs in Kaluga Region
The article provides an introductory statistics on dirofilaria on the territory of the Russian Federation. Two cases of dirofilaria in dogs in Kaluga Region which happened over the period between 2016 have been described. This shows the stationary type of infestation on this territory. Key words: Dyrofilariasis, transmissible invasion, Kaluga region, bloodsucking insects.
OBSTTETRICS, GYNECOLOGY
47 Mikhalev V.I., Lozovaya E.G., Nezhdanov A.G., Yudin S.M. Prophylaxis of intrauterine growth restriction and embryo death in cows
Comparative efficacy of application of hormonal (gonadoliberin, gonadotropin, progesterone) and nonhormonal (Sat-Som) preparations for the prophylaxis of intrauterine growth restriction and embryo death in lactating dairy cows was studied. High efficacy of complex application of Progestamag and Sat-Som preparations is demonstrated. Key words: cows, intrauterine growth restriction and embryo death, prophylaxis, Progestamag, Sat-Som.
50 Denisenko V.Yu., Kuzmina T.I. Biotesting of the donor oocytes functional status of Sus scrofa domesticus
There were monitored the parameters of fluctuations in calcium content in porcine oocytes depending on their functional status. Significant differences in indicators of the fluorescence intensity of chlorotetracycline-membrane-calcium complex in growing oocytes and oocytes that have finished growth phase at the action of hormones and nucleotides were revealed. Additional exit of Ca2+ was found in oocytes that have finished growth phase under the action of prolactin and GTP, as well as GH and GTP. In the growing oocytes the above effect was not observed. A method for biotesting the stage of the completeness growth phase of porcine oocytes was proposed based on the data obtained. Key words: oocytes of pigs, calcium.
ZOOHYGIENA, SANITATION, ECOLOGY
54 Selyaninov Y.O., Sazonov A.A., Kashkovskaya L.M. Fulgurd – a new highly effective drug for disinfection of veterinary supervision objects
The results of the approbation showed that the disinfectant Fulgurd have a high bactericidal, fungicidal, virucidal and sporicidal activity at low concentrations (0,15 – 0,5 %). A single application of 0,3% solution of drug in the amount of 0,3 l/m2 destroys the virus of African swine fever within 30 minutes. The drug may be use for prophylactic disinfection with a minimum exposure of 20 min. Key words: African swine fever, bacteria, viruses, disinfection, glutaraldehyde.
NONINFECTIOUS DISEASES
58 Grechko V.V., Khonin G.A., Sul'din E.V., Koshkin I.N., Avdeev D.B. Epidural anesthesia by Novocaine: efficacy and influence on the physiological state of rats
The effectiveness of epidural anesthesia (EA) was studied in the experiment on rats. Before it was carried, the animals were immobilized with medetomidine, and after blockade an 0,5 % solution of antimedine was injected intramuscularly to relieve the sedation. EA was performed by inoculation of 2 % solution of Novocaine in a dose of 0,5 ml between the sixth lumbar vertebra (L6) and the first sacral vertebrae (S1). A single injection of Novocaine in the epidural space provided a complete anesthesia in 75 % and partial anesthesia in 25 % of the rats. After repeated EC these rates were 90 and 10 %, and after the third EA – 60 and 40 %, respectively. One- and two-fold administration of anesthetic caused a slight deviation in the respiratory rate in animals, but during the third EA this negative effect was much more pronounced (decrease in the frequency of respiratory movements up to 75 – 90). Key words: anesthesia, novocaine, epidural blockade.
LABORATORY PRACTICE
62 Baratov M.O., Sakiribirov O.P. Improvement of the nutrient medium for the cultivation of Corynebacterium
The nutrient medium is the main factor of the quality of the bacteriological method. Therefore, it is very important when choosing the media to take into account the need of microorganisms for nutrients necessary to maintain their vital functions. A large number of nutrient media with a wide range of inorganic salts and meat hydrolysates, prepared often with a violation of the balance between the components without taking into account the growth properties, are proposed. The use of these factors introduces uncontrolled factors into the laboratory robot. For normal development, microorganisms need micro- and macro elements that participate in the formation of coenzymes, through which proteins, fats and carbohydrates are hydrolyzed to simpler and more soluble compounds available for assimilation by a microbial cell. Inorganic compounds are part of the microbial cell, cytoplasm and create the necessary physicochemical conditions for microorganisms in the nutrient medium. Key words: geothermalwater, corinebacteria, cultivation, nutrientmedia.
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