CONTENTS №12-2017

3 Michenko A.V., Semakina V.P., Michenko V.A., Karaulov A.K. Senecavirus swine vesicular disease

The article presents data on the emergent vesicular Senecavirus infection of pigs. Senecavirus was detected as a contaminant of the continious cell line. It is believed that the virus was introduced in the cell culture with fetal serum of cattle or pig trypsin. The agent is the causative agent of senecavirus swine vesicular disease and epizootic transient disease of newborn piglets. Senecavirus infection was registered in North America (USA and Canada), South America (Brazil), Europe (Italy), Asia (China, Thailand). There is a risk of bringing senecavirus into the Russian Federation with infected breed pigs, slaughter products, biological products contaminated with the virus. Key words: epizootology, senecavirus, swine, vesicular seneca virus infection of pigs, virusemia, viruses.

PRACTIC: EXPERIMENT, PROBLEMS, PERSPECTIVES

7 Donnik I.M., Voronin B.A. New in legal regulation of veterinary activities (review of normative legal acts)

The Federal Law No. 243-FZ "On Introducing Amendments to the Law of the Russian Federation on "Veterinary Medicine and Certain Legislative Acts of the Russian Federation ", adopted on July 13, 2015, introduced new concepts and directions in the field of veterinary activity. Their implementation required the adoption of by-laws. Some of them are considered in this article. Key words: veterinary science, normative legal acts, legal regulation.

11 Batrakov A.Ya., Videnin V.N., Serdiuk G.N., Ivanov Y.V. Methods of improving native Holstein half-breds cows resistance

The reasons of cattle breeding work and management practices that lead to different diseases massively affecting cows are stated in this research. Some of them, such as post-partum endometritis in 80 – 90 % of cows that just gave birth, increase post-partum interval for over 90 days and lead to a high percentage of barrenness in herds (up to 25 – 30 % and more). A high percentage of cows (up to 30 – 35 %) especially in dairy herds suffer from limbs diseases in their distal areas because of breeding native cows with Holstein cattle. This disease is associated with painfulness, low milk production and weight loss. Farms suffer from big economic losses because of areas diseases and metabolic disorders in cattle. Aforementioned diseases of dairy cows lead to early culling of cows and decrease of cow longevity (no more than 2 – 3 lactation periods). Key words: cattle, resistance in animals, post-partum diseases, limb diseases, mastitis, metabolic disorders, reproductive tract diseases, infertility, barrenness, genetic selection, life longevity.

INFECTION DISEASE

14 Glotov A.G., Koteneva S.V., Glotova T.I., Tregubchak T.V., Maksyutov R.A. Molecular epidemiology of bovine viral diarrhea virus on the big dairy farms in Siberia

Bovine viral diarrhea virus (BVDV) is considered as prototype member of the Pestivirus genus. The disease is caused by two different viruses  BVDV1 and BVDV2. Studies on genetic polymorphism of viruses in Russia insufficient. The aim of this work was a phylogenetic analysis of two types of BVDV circulating in the big dairy farms in five regions of Siberia and Northern Kazakhstan based on highly conserved region of the genome (5'-UTR). The local and imported cattle revealed the circulation of the six subtypes of BVDV1 (a, b, c, g, p, k), the presence of mixed subtypes: 1d/1n/1u and BVDV2 . Predominant subtype was 1b. The number of BVDV1 was as follows: 1a, 1c, 1d / 1n / 1u and 1p at 3,85 %; 1g – 15,38 %, 1k – 7,69 % and BVDV2 – 11,54 %. Distribution of viruses had a geographical differences. BVDV1b, BVDV1p, BVDV1g, BVDV1k and mixed subtypes 1d / 1n / 1u often detected in sick or persistently infected (PI) animals in farms with respiratory diseases. BVDV 1c was found in the serum of PI heifer without clinical symptoms. BVDV1a and BVDV2 detected in animals with reproduction pathology. Studies on the molecular epidemiology of BVDV can be used to select and optimize control strategies and measures to decide on the use of vaccines in a particular region. Key words: bovine viral diarrhea, BVDV1, BVDV2,subtypes,cattle, polymerase chain reaction, genome,5´-nontranslated region, nucleotide sequence, filogenetical analysis, molecular epidemiology.

21 Batomunkuev A.S.,Anganova E.V., Adlov A.M., Pliska A.A., Trofimov I.G.

The antibiotic resistance of Enterobacteriaceae and Staphylococcus isolated from dogs in the Irkutsk region

The analysis of antibiotic resistance of 30 strains Enterobacteriaceae (pathogenic Escherichia coli, opportunistic Enterobacteriaceae) и 10 strains Staphylococcus isolates from the dogs in the Irkutsk Interregional Veterinary Laboratory was performed. The pathogenic E. coli were resistant to β-lactam antibiotics (carbenicillinum, cefotaxime, ceftazidime, cefalotin) and sensitivity to amikacinum, gentamicinum, kanamycinum, ofloxacinum, ciprofloxacinum. Оpportunistic Enterobacteriaceae sp. were resistant to β-lactam antibiotics (carbenicillinum) and have a wide (as compared to pathogenic E. coli) spectrum of resistance to cephalosporinums (cefalotin, cefotaxime, ceftriaxonum, ceftazidimum, cefazolinum), but were sensitive to gentamicinum and ofloxacinum. Staphylococcus demonstrated a high level of resistance to penicillinum, cefazolinumum, cephalexinum, erythromycinum, roxithromycinum, lincomycinum and sensitivity to oxacillinun and ciprofloxacinum. 17,4 % pathogenic E. coli and 14,3 % opportunistic Enterobacteriaceae were susceptible to all antibiotics used in the work. Among isolates pathogenic E. coli 47,8 % had phenotypes resistant to drugs of 2 classes, 28,6% isolates opportunistic Enterobacteriaceae – to drugs of 2 and 4 classes. Proteus vulgaris and S. aureus had the largest spectrum of antibiotic resistance (to drugs of 6 classes). MDR phenotype recognized in all cultures of Staphylococcus, in 21,7 % isolates of E. coli and 42,9 % isolates of opportunistic Enterobacteriaceae. Key words: antibiotic resistance, infectious diseases, E. coli, dog,

24 Yaralova E.A., Kozhushkevich K.A., Biryukova A.Y., Yatsentyuk S.P., Obukhov I.L. Genetic diversity of feline coronaviruses from Moscow region

The distribution of Feline Coronaviruses type I and II in Moscow region were analyzed in PCR. The nucleotide sequences of the S gene of the viruses isolated from animals from households and from private owners were compared. Key words: Feline Coronavirus, PCR, nucleotide sequencing, phylogenetic analysis.

INVASIVE DISEASES

28 Safiullin R.T., Ustinov A.M. Gelmicidum and Fascocidum with spontaneous fascioliasis of cattle

Scientific and industrial trials have shown that with a high intensity of fasciolous invasion of cattle, the recommended dose of the complex preparation Gelmicidum 7,5 g for every 100 kg of animal mass is once inwardly ineffective – EE – 80 %, IE – 83,8 %. In this case, it is quite justified to increase the therapeutic dose of the drug by 13 % and assign 8,5 g of the preparation for every 100 kg of the animal's mass, which provided a high extent and intensity (EE – 100 %).

The results of the production test confirmed the previously obtained data on the effectiveness of the Gelmicidum preparation in the optimal dose of 8,5 g per 100 kg of body which corresponds to a dose of 5,95 mg/ kg oxyclosanide and 17 mg/ kg albendazole according to the active substance we established once, 96,7 % EE and 97,7 % IE were obtained. The recommended dose of 10 mg / kg body weight with feed once in the production conditions was 86,7 % EE, and 93,8 % IE. The economic effect per cow per day compared to the base variant in the first group (Gelmicidum) was 85,9 rubles; On the second (Fascocidum) group – 46,5 rubles. The total economic effect on the group of cows during the experience after treatment (3 months) compared to the base variant in the first group (Gelmicidum) was 154,6 thousand rubles, for the second group (Fascocidum) – 83,7 thousand rubles. Key words: cattle, fascioliasis, infection, diagnosis, treatment, anthelmintics, Gelmicidum, Fascocidum, Santel, medical and economic efficiency.

33 Akbaev R.M. Method of evaluation the effectiveness of insectoacaricide’s dusts

There are described the method of in vitro evaluation effectiveness of insectoacaricides in the form of dust on the example of the most common ectoparasite of agricultural poultry – red chicken mite Dermanyssus gallinae. Key words: dust, insectoacaricide, mites, Dermanyssus gallinae.

37 Shakarboev E.B., Golovanov V.I., Safarova F.E., Kuznetsov D.N. The helminths of grass carp and silver carp live in northeast of Uzbekistan

439 grass carps and 498 silver carps from the natural ponds and fish farms in northeast of Uzbekistan were investigated for helminths. There were detected 12 species of helminths in grass carps and 13 species of helminths in silver carps. The increasing of species diversity of helminths parasitizing in fishes in Uzbekistan indicates the necessity of conducting preventive measures in Republic. Key words: aquaculture, fish-farming, grass carp, silver carp, helminths.

OBSTTETRICS, GYNECOLOGY

40 Kuzmina I.Yu., Lykov A.S. Effect of Rhodiolae rozea and laminaria on physiological state and reproductive functions of cows

The studies were carried out on use of feed additive (Rhodiolae rozea L, laminaria, trace elements) in diets for cattle feeding. The use of the feed additive promotes increasing average daily milk yield by 7,39 %, fat content in milk by 0,1% relative to the check values, positively influences on a physiological state and reproductive function of cows. Cows fed the feed additive have a service-period shorter by 28,3 days, insemination index lower by 0,5, impregnation rate after the 1^st insemination higher by 20 % as compared with a check cows group. Key words: cattle, feed additive, Rhodiolae rozea L., laminaria, trace elements,milk productivity, biochemical and morphological indices of cattle blood,reproductive function.

44 Filatov A.V., Ushakova L.M., Lobanov V.S., Khlopitskiy V.P. Efficiency of application of preparation Progestamag^®to improve the reproduction of breeding stock of pigs

Efficiency of hormonal preparation Progestamag^®for increase fertilization and it is a lot of fruitis the basic sows and repair pig Is studied. It is established, that exogenous introduction of a progesterone in early gestation causes higher endocrine the status of animals to what increase of concentration of steroid hormones in blood and increase of reproductive indicators testifies. Key words: Progestamag^®, endocrine the status, embryonal death rate, a reproduction sows.

NONINFECTIOUS DISEASES

48 Lemeshchenko V.V., Kuzina N.S., Skobel'skaya T.P. Features of provisoral structures of lungs and liver in lambs|eanling| determining them adaptogenesis on neonatal stage

LABORATORY PRACTICE

53 SelimovR.N., DzhatdoevaA.A., MetalnikovP.S., KomarovA.A.Determination of mycotoxins content in feeds and feed raw materials by high-efficient liquid chromatography with a mass-spectrometric detector

This article is devoted to the analysis of mycotoxin contamination in samples of feed and raw feed components, which were received by the FGBU “VGNKI” in 2014 – 2016. The occurrence and content of a wide range of mycotoxins (48 names of mycotoxins) in 476 samples of feed and feed material were analyzed by high-performance liquid chromatography with a mass spectrometric detector. The study showed that 72 % of the samples were contaminated with mycotoxins. The percentage of mycotoxins-positive samples was 91 % for combined feed, 50 % for raw feed components. The most common mycotoxins were the toxins of Alternaria spp. fungi: tenuazonic acid was found in 197 (41 %) of the analyzed samples, tentoxin was found in 141 (30 %) samples, alternariol in 76 (16 %) samples, alternariol methylether in 48 (10 %). HT-2 toxin was detected in 51 (11 %) samples. Six samples contained mycotoxins at concentrations exceeding maximum permitted levels set in legislative acts of Russian Federation. Key words: feeds, feed raw materials, mycotoxins, HPLC-MS.

59 FROM VETERINARY MEDICINE HISTORY

64 Index of articles, published in 2017

CONTENTS №12-2017