CONTENTS №4-2021

3 Mukovnin A.A., Naimanov A.Kh., Tolstenko N.G., Vangeli E.P. Paratuberculosis of cattle in the Russian Federation

For the first time, the article presents an analysis of the epizootic situation in cattle paratuberculosis in the Russian Federation for 1991-2019. It was found that earlier, and over the last 29 years of the analyzed period, there was no widespread paratuberculosis in Russia. So, the number of unsuccessful points ranged from 2 to 6 per year, patients with paratuberculosis, animals with a "clinical" form were identified in isolated cases, paratuberculosis disease did not become widespread. The authors believe that these facts are associated with the chronic course of the disease and the implementation of health-improving measures: regular culling of animals with a "clinic" of paratuberculosis, as well as old and low-productive animals. In addition, in dysfunctional farms, culling and slaughter of animals with positive reactions to serological and allergic tests is carried out. Key words: paratuberculosis, cattle, epizootological analysis, unfavorable points, clinical form, latent course, small intestine, bacteriological method, PCR method.

DOI:10.30896/0042-4846.2021.24.4.03-07

EXPERIMENT, PROBLEMS, PERSPECTIVES

8 Sobolev V.A., Soboleva G.Yu., Parkhomenko S.A., Zeynalov O.A. The effectiveness of the drug Dogferon® in puppy parvovirus

A complete blind, placebo-controlled, randomized, prospective study of the efficacy and safety of the veterinary drug Dogferon® in puppies with parvovirosis was carried out. Animals (n=20) admitted to the veterinary center underwent clinical and laboratory examination. After randomization, the puppies were treated. The experimental samples of the drug were depersonalized. The condition of the animals was assessed daily. Blood tests (general and biochemical analyzes) were carried out on the 1st and 5th day of the experiment. Deciphering of the prototypes of the preparation was carried out as part of the commission upon completion of the complete analysis of the data obtained. The inclusion of the drug Dogferon® in the treatment regimen for parvovirosis facilitates the course of parvovirosis, reduces mortality (p˂0,05), and accelerates the time of clinical recovery of animals (p=0,016). Key words: Dogferon®, canine parvovirus, interferon alpha canine, puppies.

DOI:10.30896/0042-4846.2021.24.4.08-11

12 Svintsov R.A., Razzhivin A.V., Usachev E.A., Abramova E.G., Nikiforov A.K. Hematology analyzers of hematological parameters of producing animals in the production of medicines from

To monitor the hematological parameters of producers in the production of drugs from animal blood, the possibilities of modern veterinary hematological analyzers of class 3-diff, which determine more than 20 different indicators, are considered. The entire volume of hematological information is analyzed-indicators related to white blood cells, red blood cells and platelets. From the leukocyte group, the number of white blood cells (WBC), lymphocytes (LYM), medium cells (MID), and granulocytes (GRA) were considered. From red blood cells: the number of red blood cells in the blood (RBC), hemoglobin content (HGB), hematocrit (HCT), red blood cell indices (MCV, RDW-CV, RDW-SD, MCH, MCHC). Platelet count: the number of platelets in the blood, thrombocrit and platelet indices (MPV, PDW, P-LCR). The definition of each indicator, its technical characteristics, functional value, possible and most likely diagnostic scenarios when indicators go beyond the normal values are given. In addition to numerical indicators, the hematological analyzer draws histograms of the volume distribution of white blood cells, red blood cells, and platelets. Characteristics of each of the 3 possible histograms, diagnostic approach to graphical data, and interpretation of their signals are given. Key words: monitoring of hematological parameters, producing horses, hematological analyzer, histogram, white blood cells, red blood cells, red blood cell indices, platelets, platelet indices.

DOI:10.30896/0042-4846.2021.24.4.12-17

INFECTION DISEASE

18 Chadaeva A.A., Povolyaeva O.S., Kolbasova O.L., Lapteva O.G., Dmitrenko V.V., Morgunov Yu.P., Lunitsin A.V., Yurkov S.G. Biological properties of goat kid testicle cells subculture

The paper presents the results of studies on the creation of a cryobank of goat kid testicular cells subcultures, which are standardized by cytomorphological, growth characteristics and by the absence of contaminates, including ruminant lentiviruses. The results of the assessment of their permissivity to peste des petits ruminants virus, lumpy skin disease virus, and Aujeszky's disease virus demonstrates that this cellular system can be used for the isolation of field strains of these viruses. The sensitivity of this cell culture to the vaccine strain of classical swine fever allows us to consider the obtained cells as a reserve for biotechnology purposes. Key words: cell cultures, viruses, peste des petit ruminant, lumpy skin disease, Aujeszky's disease.

DOI:10.30896/0042-4846.2021.24.4.18-24

25 Goncharov A.T., Lenev S.V. Prevention and treatment of avian salmonellosis with monoglycerides

The paper presents data on the prophylactic efficacy of a new class of antibacterial drugs, alternative to antibiotics, based on 1-monoglycerides, during experimental infection of broiler chickens with salmonellosis. The use of the antibacterial drug SalcoliTM MonoBP in the experimental infection of broiler chickens with a virulent strain of Salmonella allows them to protect them from salmonellosis and sanitizes their body from the causative agent of salmonellosis, after 2-3 weeks of daily use. A biological product based on 1-monoglycerides of fatty acids has a noticeable growth-stimulating effect, contributing to an increase in average daily weight gain in broilers by 5.0-5.5% and an improvement in feed conversion by an average of 6% compared to control. Key words: salmonellosis, Salcoliтм MonoBP, monobutyrin, monopropionin, Salmonella enteritidis, poultry, monoglycerides.

DOI:10.30896/0042-4846.2021.24.4.25-29

29 Skrynnikova T.I., Kuznetsova M.I. Preventiv and curative effect of the probiotic Belolin and Vetom 1 for salmonellosis in experiment on white mice

The article reflects the results of the study of the preventive and therapeutic effects of the probiotics Belolin and Vetom 1, which are a suspension of spore material of the strains Bac. subtilis and Bac. licheniformis, in experimental salmonellosis of white mice. It is shown that both drugs have a pronounced therapeutic and preventive effect. Key words: probiotics, Belonin, Vetom 1, salmonellosis, strain, microorganisms, lymphocytes, prevention, treatment.

DOI:10.30896/0042-4846.2021.24.4.29-32

INVASIVE DISEASE

33 Kovalchuk S.N., Arkhipova A.L. The distribution of bovine anaplasmosis in the Russian Federation

Bovine anaplasmosis is a tick-borne infectious disease that causes significant economic damage to livestock farms around the world due to the loss of milk and meat production and the death of livestock. In the Russian Federation the nosoareal of bovine anaplasmosis has significantly expanded over the past decade primarily due to a change in the habitat of ticks in the context of global warming. This article presents data on the prevalence of bovine anaplasmosis in the farms of the Samara region and Primorsky krai, as well as results of molecular genetic typing of the Russian isolates of Anaplasma marginale and A. ovis, the causative agent of anaplasmosis in cattle and small ruminants. Key words: anaplasmosis, Anaplasma marginale, A. ovis, cattle, genotyping.

DOI:10.30896/0042-4846.2021.24.4.33-37

37 Nikochenko T.S. Species composition of mites, vectors for vector-borne diseases, meets the dogs in the Kuzbass

The article analyzes ticks (species composition) found on dogs in Kuzbass for the period 2018 – 2020. The natural and geographical conditions of the Kemerovo region are described. Ticks were collected from dogs for 2018, 2019 and 2020. 228 dogs took part in the experiment and 387 ticks were collected. The main ticks harvested from dogs, are of the following species: Dermaceptor reticulatus, Ixodes persulcatus, and Ixodes pavlovskyi. Conclusions and proposals were formed. Key words: babesiosis, ixodids, ticks, Ixodes persulcatus, Ixodes pavlovsky, Dermaceptor reticulatus, dogs, Kuzbass.

DOI:10.30896/0042-4846.2021.24.4.37-40

PHARMACOLOGY AND TOXICOLOGY

41 Engashev S.V., Shemyakova S.A., Aliev M.A., Kolesnikov V.I. Tolerance of the drug Flyblock® insecticide tag by calves at pasture

The tolerability of the drug Flyblock® insecticide tag in the form of a polymer tape containing s-fenvalerate and piperonyl butoxide as active substances in calves was studied. The drug does not have a negative effect on the general condition of calves, activity, feed and water consumption, the functional state of the organs and systems of the body of calves, hematological and biochemical parameters, the dynamics of weight gain of calves after using the drug in a therapeutic dose (a tag on each ear). The active substances of the drug throughout the entire period of use are not absorbed into the blood and do not accumulate in the organs and tissues of the animal. Key words: drug Flyblock®, tag, tolerance, calves, hematological and biochemical parameters, body weight dynamics, safety.

DOI:10.30896/0042-4846.2021.24.4.41-46

46 Grozina A.A., Vertiprakhov V.G., Kislova I.V., Ovchinnikova N.V., Koshcheeva M.V. Alterations in the hematological and biochemical blood parameters in broilers induced by the experimental mycotoxicosis

The effects of experimental T-2 toxicosis on the morphological and biochemical blood parameters were studied on four treatments of broiler chicks (cross Smena-8, 30 – 50 days of age, 10 birds per treatment) with similar initial live bodyweight in 2020. Control treatment 1 (intact control) was fed diet without T-2 toxin; experimental treatments 1, 2, and 3 were fed diets supplemented with T-2 toxin in concentrations 1, 2 and 4 maximal allowable levels (or 0,1; 0,2 and 0,4 mg/kg), respectively. It was found that all doses of T-2 toxin negatively affected the morphological and biochemical blood parameters: concentration of leukocytes in the intoxicated birds was lower by 5,8 – 18,9 % in compare to intact control, neutrophils by 8,8 – 35,1 %, monocytes 2 – 6-fold, eosinophils 2 – 3-fold, while concentration of basophils was 2 – 4-fold higher, lymphocytes higher by 9,5 – 52,0 % in compare to control, depending on the dose of the toxin and exposure period (the duration of consumption of infected feeds). The concentration of glucose in blood serum of the intoxicated birds changed abruptly and unevenly, with increases by 15,3 – 19,2 % and decreases by 9,9 – 17,4 % in compare to control; the activity of trypsin in serum decreased by 31,7 – 35,9 %, depending on the dose of the toxin and exposure period. The phosphatase-protease index (ratio of the activities of alkaline phosphatase to total proteases) in serum increased by 17,4 – 18,1 % in compare to control after 1 week of exposure to contaminated feeds; after 2 weeks of exposure the increases were 29,3 % in treatment 2 and 233,5 % in treatment 3 indicating serious metabolic disturbances in birds with severe experimental T-2 toxicosis. Key words: broilers, mycotoxins, biochemical blood parameters.

DOI:10.30896/0042-4846.2021.24.4.46-52

LABORATORY PRACTICE

53 Plotnikova E.M., Nesterova I.A., Nizamov R.N., Nefedova R.V., Sayfullin A.S., Pankova E.V. Immunochemical assesment of the contamination level of cell cultures applied in biotechnology

In the present study we developed method enzyme immunoassay (ELISA) for the detection in extraneous microflora contaminants of cell cultures. ELISA modifications using lg G-200 conjugate and high-resolution liquid chromatography were used to quantify whole microflora cells that are most commonly encountered in animal cell culture in vitro. Some regularities of adsorption of bacterial cells (mycoplasmas) on the surface of transparent polystyrene and the reaction of antibody interaction with immobilized cells were studied. The analysis conditions are optimized. Calibration curves were obtained by using the sandwich method. Our results showed that allow determining 3000 cells of foreign microflora in ml using. The analysis time was 2 – 3 hours. Key words: mycoplasma, enzyme-linked immunosorbent assay, virusvaktsinu, radioaktiven, contaminant.

DOI:10.30896/0042-4846.2021.24.4.53-57

57 Novikova N.N. Use of colored brucellosis antigens in the micro-reaction of agglutination

The article discusses the possibility of using colored brucellosis antigens when staging an agglutination reaction using the micromethod. On the example of blood serum of guinea pigs immunized with vaccine strains of Brucella, which are in different antigenic forms S-, RS-, R. As a result of the studies carried out, it was found that both methods have sufficient sensitivity to diagnose brucellosis. At the same time, in the differential diagnosis of infection from post-vaccination reactions, it is more convenient to use colored R, S-antigens for RMA, since this method is 2 times more sensitive than traditional RA. Key words: brucellosis, agglutination reaction, antigens, sera, guinea pigs.

DOI:10.30896/0042-4846.2021.24.4.57-59

60 FROM VETERINARY MEDICINE HISTORY