CONTENTS №7-2019

3 Tyrin V.G., Mysova G.A., Biryukov K.N. Environmental protection at livestock enterprises

The article discusses the problem of environmental pollution in the area of livestock enterprises activity. There are shown the main sources and levels of air, soil and water pollution on the livestock farms and industrial-type complexes, as well as measures aimed at reducing emissions of pollutants into the environment. Key words: environment, pollutants, manure, litter.



9 Filatov A.V., Sapozhnikov A.F., Khlopitskiy V.P. Pulmagag®  for the  treatment of calves with respiratory disease

Efficiency of a new complex preparation Pulmamag® is shown, the basic operating which substances are an antibiotic azithromycin and not steroid resolvent meloxicam at treatment of the calfs sick sharp is purulent-kataralnoj bronchopneumonia. Distinctive feature of the preparation offered for wide application Pulmamag® in a complex with other means of symptomatic therapy is faster improvement of the general condition of animals, positive influence on hematological a profile and the short period of rehabilitation of young growth of large horned livestock. The preparation Pulmamag® is recommended to be applied at complex therapy once a day within 3 – 5 days in a dose of 0,5 ml on 20 kg of weight of an animal. Key words: a pathology of bodies of breath, bronchopneumonia, calfs, Pulmamag®, treatment.

14 Rudenko P.A. The role of intestinal dysbacteriosis in the mechanisms of the formation and progression of surgical infection in cats

The article presents data on the role of intestinal dysbacteriosis in the mechanisms of the onset and development of accidental purulent-inflammatory processes of soft tissues in cats. It is shown that in the formation and progression of surgical infections in cats, dysbacteriosis of the intestine arises and intestinal translocation of opportunistic pathogens into the focus of purulent inflammation is evidenced by the determination of the antigenic kinship of cultures of intestinal microorganisms and wound sites according to agglutinable properties, as well as the comparison of antibioticograms of microorganisms isolated from cats from a soft tissue biopsy and fecal samples with an operating wound and with different forms of surgical infection . Depending on the severity of the surgical infection, the microbiota changes in the intestine, and in the group of animals with sepsis they approach critical values, which indicates the development of irreversible reactions in the body. Key words: dysbacteriosis, ovariohysterectomy, purulent wound, abscess, sepsis, cats.



21 Nefedeva M.V., Titov I.A., Malogolovkin A.S.  Porcine epidemic diarrhea virus recombinant and its genetic characteristics

The Porcine epidemic diarrhea virus (Coronaviridae family) is one of the causes of debilitating diarrhea and severe organism dehydration. PED causes significant economic damage to the pig industry, which indicates the need to study the pathogen biology and the development of prevention tools. Currently, not enough knowledge has been accumulated about the genetic characteristics of the complete genomes of the PEDV isolates isolated in the Russian Federation territory. In this study, we present the analysis results of the PEDV/Belgorod/dom/2008 isolate complete nucleotide sequence of the PEDV genome. Key words: family Coronaviridae, Porcine epidemic diarrhea virus, recombination, sequencing, phylogenetic analysis.


27 Feodorova V.A., Saltykov Y.V., Filonova N.N., Subbotina I.A., Zaitsev S.S., Ulianova O.V.,  Larionova O.S., Evstifeev V.V., Revzina E.M., Ulyanov S.S.,  Motin V.L. Detection of the novel swedish variant of Chlamydia trachomatis in cattle

The DNA of Chlamydia trachomatis (CT) of the new «Swedish» variant (nvCT) with a deletion of 377 b.p.  in the orf1 gene of a cryptic plasmid was detected in clinical specimens from aborted cows. All of them had diagnostic titers of specific chlamydial antibodies in compliment fixation test. Molecular omp1 typing demonstrated the complete homology of the nucleotide sequences of the bacterial strains identified with the CT genovar E (variants of the E1 subtype: E/Bour, Sweden2, Sweden3). Genetic material (DNA) of other Chlamydia species, including Chlamydia psittaci and/or Chlamydia abortus, as well as other potential causative agents of infectious abortions of livestock (Campylobacter spp., Listeria spp., Toxoplasma gondii, Salmonella spp., Brucella spp., etc.) in the specimens studied by real-time PCR commercial kits was not detected. Key words: Chlamydia trachomatis, chlamydial abortion, livestock, cattle, PCR, novel Swedish variant.


31 Imatdinov A.R., Kazackova A.S., Morozova D.Yu.,  Lyska V.M.,  Zhivoderov S.P., Sereda A.D. Experience gained in preparation of hemadsorption inhibiting sera against African swine virus seroimmunotypes III and IV

This paper presents the results of determination of optimal conditions for obtaining hemadsorption inhibiting antisera against African swine fever virus (ASFV) seroimmunotypes III and IV. A preliminary injection of a minimum protective dose of an attenuated hemadsorbing strain followed by infection with a homologous virulent ASFV reference strain together with phosphonoacetic acid (FUC) injection to reduce the probability of death from the disease was shown to be the most effective approach here. Thus, serotype III and IV antisera active in haemadsorption inhibition (HAdI) tests with titers or 1:80 to 1: 640 were obtained. Key words: African swine fever, serotype-specific sera, hemadsorption inhibition test.



38 Karmaliyev R.S., Akhmedenov  K.M., Sidikhov B.M., Aytuganov B.E., Usenov Zh.T., Ertleuova B.O., Gabdullin D.E., Aliyev E.M. Seasonal and age dynamics of helminths invasion level of cattle in various natural zones of West Kazakhstan region

Studies have shown that сattle infected by M. expansa, E. granulosus, T. rhodesi and strangulates of the digestive tract in all natural areas of West Kazakhstan region. Differences of parasitizing of these helminths in the animals of different age groups and in different seasons were revealed. Key words: age, helminths, West Kazakhstan region, cattle, natural zones, seasonal.


42 Nikolenko T.S., Zubova T.V. Experience of treatment of babesiosis of dogs on the basis

In article various methods of treatment of babesiosis of dogs are considered. Studied the blood smears of patients with babesiosis in animals. An effective dose of pyro-stop in the treatment of the disease was revealed. Key words: Babesiosis, symptoms of babesiosis, diagnosis, treatment methods, Pyro-stop and Gamavit preparations.



45 Naumenkova V.A. Influence of collateral effects of vaccination on stallions’ semen

Vaccination being the strong stress-factor has the strong influence on the different systems of organism. The question of the influence of immunoprophylaxis of infections diseases on reproductive functions of animals, especially stallions, practically stays open. Studied investigation showed that vaccination of stallions against dermatomicose  by vaccine Polyvak-TM decreased the quality of semen, especially mobility and surviving after freezing- thawing process. It is important to take into account this fact when cryoconservation of semen. Rehabilitation of the quality of fresh semen happens not earlier than in 25 days after the first vaccination, for deconserved semen – not earlier than in 55 days. Key words: stallions, sperm, Polivak-TM vaccine, cryoconservation.



49 Kuznetsov S.S.  An innovative approach to preparing the pig complex

Disinfection on pig farms is the key to healthy livestock, from which at the end of the production cycle it will be possible to obtain high-quality products. The main tasks which are solved by complex washing and disinfection, pig-breeding enterprises:  control of pathogens that cause swine diseases; destruction of rodents and insects; ensuring appropriate conditions for the maintenance of agricultural animals. This article presents the results of successful application and provides recommendations on the use of products for washing and disinfection – Ecocline ALK f, MS Hyperdez, MS Aza fly, MS Maggot.  Key words: disinfection, sanitation, detergents, microbial resistance, insecticidal program.


52 Kochish I.I., Maksimov V.I., Shukanov R.A., Shukanov A.A., Lezhnina M.N.  Postnatal formation immunophysiological status of pigs in the heliogeophysical conditions of the region

The linear character of postnatal variability of indicators of natural resistance and productivity in pigs of both experimental and intact groups is revealed. This fact testifies to almost identical regularity of improvement of the immunophysiological status in experimental animals in the phases of newborn, milk type of feeding, sexual and physiological maturation, regardless of the studied environmental factors. Key words: pigs, trepel, Suvar, Polistim, immunophysiological status, phases of postnatal ontogenesis.



56 Zakharychev V.V., Brevnova B.O., Kuzenkov A.V., Komaritskikh M.Yu., Trubnikova O.N. Developement of HPLC-MS/MS method for the analysis of free lipophilic biotoxins in mollusks

There are no any valid methods in Russia for the determination of lipophilic biotoxins in molluscs compliant with the European Union regulations. The fact makes great hindrance for shellfish export. We have developed reverse-phase chromatographical conditions using acidic buffer as a mobile phase and tandem mass-spectrometric detection parameters for nine biotoxins: okadaic acid (OA), dinophysis toxins 1 and 2 (DTX1 and 2), pectenotoxin 2 (PTX2), yessotoxin (YTX), homoyessotoxin (homoYTX), and azaspiracids 1 – 3 (AZA1 – 3). For OA, PTX2, YTX, AZA1 a 3-days validation procedure was performed using the Comission Decision 657/2002/EC as guideline with fifty four samples of tissues of oysters, mussels and scallops fortified with 0,5; 1; 1,5 times of the EU permitted limits of the biotoxins. Linear response in the investigated concentration range was observed; the recovery was within 97 – 108 %, the intermediate precision values were always lower than 8.5 %. The values of decision limit CCα amounted to 174 μg/kg for OA, 170 μg/kg for PTX2, 1083 μg/kg for YTX, 170 μg/kg for AZA1. The limits of detection and limits of quantitation for these four biotoxins were estimated, as well as expanded uncertainties in measurements. The procedure is highly sensitive and selective enough to determine isomeric OA and DTX2 separately. The suitability of procedure for routine analysis of lipophilic marine biotoxins was shown. Key words: azaspiracids, validation, dinophysis toxins, high performance liquid chromatography, marine biotoxins, mass-spectrometry, okadaic acid, pectenotoxins, phycotoxins, yessotoxins.



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CONTENTS №7-2019