CONTENTS №8-2019

3 Barkova A.S., Elesin A.V., Nezhdanov A.G., Klimov N.T.  Teats hyperkeratosis in dairy cows

The paper presents findings on the incidence of teats hyperkeratosis in lactating cows at high-producing dairy herds and its role in the development of mastitis. The clinicopathologic manifestation of the disease is characterized; the concept of its etiology and pathogenesis is substantiated. Environmentally friendly specific pharmacologic drugs for treatment and prevention are offered. Key words: caws, mammary gland, hyperkeratosis, organosilicon glycerohydrogel, probiotics.



8  Vasilevich F.I., Bachinskaya V.M., Deltsov A.A. Efficiency of application of protein hydrolysates in poultry farming

Introduction of protein hydrolysates - feed additives to the diet of broiler chickens – Abiopeptide and Ferropeptide in a dose of 1 ml/kg and water-soluble in a day after a 5-day-old age to 35 days of age, does not adversely affect the clinical condition of the poultry and is cost-effective. Promotes an increase in live weight with the application of Abiopeptide by 4,1 %; when applying the ferropeptide by 5,2 % in relation to the control. Increases the gross increase in the first trial by 2,380 kg and 3,045 kg respectively. Increases carcass yield, which is 85,71% and 86,54 % respectively, and in the control 85 %. In connection with the foregoing, it is possible to recommend the use of protein hydrolysates in the diet of broiler chickens to stimulate metabolic processes and increase productivity. Key words: aminoacids, quality, safety, poultry farming, protein hydrolysates.


12 Khlopitskiy V.P., Filatov A.V., Minin A.V. Clinical estimation of an urgency of application complex intrauterine means for preventive maintenance and treatment of sharp postnatal illnesses at sows

In article results of research-and-production researches on an urgency and efficiency of application complex intrauterine devices means for preventive maintenance and treatment of postnatal illnesses at sows are presented. Optimum doses and intervals of introduction of preparations of a series Endometramag® are defined. Safety of preparations is established at an estimation of a clinical condition of the sows, confirmed hematologic, morphological and immunobiochemical by blood indicators. High preventive and medical efficiency is shown at postnatal diseases and positive influence of preparations on contractile function myometrium with the expressed and prolonged action is defined. Positive dynamics after application of preparations on viability, safety and productive qualities of pigs is tracked. Key words: sows, a postnatal pathology, sharp it is purulent-kataralnyj endometritis, symptom complex a metrit-mastitis-agalaktija, Endometramag-K®, Endometramag-T®, Endometramag-Bio®, Endometramag-Grin®, Меtramag®.


18 Stafford V.V., Streltsova E.B., Raev S.A., Yushakov A.G., Zaberezhny A.D.Aliper T.I.  Application of immunohistochemistry method in diagnosis of porcine circovirus associated diseases

Porcine circovirus associated diseases (PCVAD) remain one of the urgent problems in pig industry. Porcine circovirus type 2 is a causative agent of the disease that has an ubiquitous distribution among pigs. In the absence of pathognomonic signs of the disease, laboratory diagnostic, in particular, immunohistochemistry (IHC) is required to establish the diagnosis – PCVAD. The present study describes the visualization of viral antigens based on development of the IHC method for detection of PCV-2 capsid protein in pathological material. As a result of this work, it was found that the developed IHC method allowed to establish a relationship between the presence of PCV-2 and pathologic observations. Key words: PCVAD, PCV-2, immunohistochemistry, monoclonal antibodies, capsid protein.


22 Vlasov M.E., Sereda A.D., Balyshev V.M.  African swine virus maintenance in stable flies (Stomoxys calcitrans) and blowflies (Calliphoridae)

The heparinized virus-containing blood of pigs, infected with the virulent strain Stavropol 01/08 of ASF virus, was being fed in the laboratory to stable flies and blowflies. As show the research results of suspensions from flies using PCR-RV, and the extraction of the agent of ASF in cell culture, virus persisted in the flies for at least 5 days. After intramuscular injection the homogenates of stable flies and blowflies, that feeding virus-containing blood, two pigs were infected on the 7th and 10th day. The disease was followed with typical symptoms and resulted in death on 12th and 15th day. Autopsies revealed pathological changes typical for this infection. The results suggest that these flies can be vectors of ASF. This is indirectly confirmed by the increase in the number of ASF outbreaks in domestic pigs and wild boars in the summer and autumn period. Key words: African swine fever, hemadsorption, flies, infectious activity, viral genome, pigs.



26 Shibitov S.K., Petrova O.V., Bazhibina E.B., Motskevichus A. Chemotherapy of dogs lambliosis

The efficacy of drugs with active substance febantel (Dirofen®, Prazitsid® Suspension Plus and Drontal® Plus) was evaluated in experimental lambliosis of dogs. All tested drugs were highly effective. Key words: giardia, giardiasis, dog, febantel, chemotherapy.


31 Kashkovskaya L.M., Orobets V.A. Sensitivity of area isolates of eimeria to new coccidiostatics

As a result of the research found, that anticoccidial drugs: Melasit 40 %, Nicarzin, Coccimax, Decvycox and Eimicid show good coccidiostatic activity to area isolates of eimeria. The inclusion of these funds in the anticoccidian program will allow you to save livestock and increase bird productivity. Key words: eimeriosis, broiler chickens, anticoccidial drugs.


34 Filippova O.B., Saranchina E.F., Krasnoslobodtseva A.S.  Medicinal plant for new-calved cows

The possibility of use the wild plants Artemisia vulgaris L. and Salvia pratensis L. as a prophylactic and stimulating feed additive in the rations of newly calved cows was studied. It was found that the feeding of these phyto-additives during the first 4 – 5 weeks after calving increases the milk productivity of cows by 9,8 %, protein content in milk by 6,5 % and its density. Milk fat content corresponded to the level of metabolism, which was more balanced in the animals receiving the feed additive than in the control cows, which were given only the basic ration. The bactericidal effect of herbal supplements made it possible to exclude the occurrence of clinical mastitis and to reduce the number of somatic cells in the milk of healthy experimental animals by 27,8 % in comparison with healthy control cows. Key words: cows, mastitis, medicinal herbs, milk quality, productivity.


38 Chistiakova I.V., Kuzmina T.I., Denisenko V.Yu. The evaluation of the bovine spermatozoa viability with induced capacitation before and after thawing

It has been shown that the preliminary capacitation of bovine spermatozoa before freezing increases the number of viable spermatozoa after thawing [2]. In artificial insemination, thawed spermatozoa are transferred to the female reproductive tract, where the capacitation occurs. In this study, we examined the effect of conditions, which provide the passing of capacitation on thawed spermatozoa, preliminarily capacitated before freezing. It has been shown that the incubation of the control thawed (without prior capacitation) spermatozoa in the absence, as well as in the presence of dbcAMP and IBMX, prolactin (PRL) and GTP for 4 hours did not lead to the change in the ratio of live/dead cells. Incubation of thawed spermatozoa, which had been capacitated previously before freezing, during 4 hours with dbcAMP and IBMX, PRL and GTP, led to an increase in the number of viable spermatozoa. The usage of chlortetracycline probe (determining the location of the fluorescence of chlortetracycline in spermatozoa) showed that in intact thawed spermatozoa the ratio of cells with different functional status - uncapacitated, capacitated and acrosome-reactive, was similar among all experimental groups. In thawed spermatozoa, which had previously capacitated before freezing, the decrease in the number of capacitated and the increase in the number of acrosome-reactive cells in the presence of dbcAMP and IBMX, and also PRL and GTP were noted. Thus, the second incubation of thawed spermatozoa for 4 hours, which had previously been capacitated before freezing, did not increase the number of viable cells. Key words: capacitation, freezing, bovine spermatozoa.



42 Grachev S.Yu., Zubova T.V. Increasing the intensity of growth and safety of newborn calves by pharmsubstania of Rhapónticum carthamoídes

The experiment established the effectiveness of the pharmaceutical substance Rhapónticum carthamoídes and determined the optimal dosage of the preparation for 14 – 28-day old calves of black-and-white breed. Daily feeding it during this period provided an increase in the intensity of growth and safety of the animals. Key words: calves, medicinal plants, prevention, pharmaceutical substance.


45 Prusakov A.V., Zelenevskiy N.V., Shchipakin M.V., Plemyashov K.V., Bylinskaya D.S.  Ultrastructure of hen hematoencephalic barrier

The aim of the work is to study the ultrastructure of the tissue organization of the blood-brain barrier of the cross White Loman. There were characteristic for it close contacts between endothelial cells, small number of pinocytotic vesicles in the cytoplasm of endothelial cells, the presence of basal membrane, dublicator containing pericytes and presence of astrocytic solid sheath around the vessels. Key words: brain, blood-brain barrier, bird, blood system, electron microscopy, ultrastructure.



49 Smirnova I.R., Satyukova L.P., Shopinskaya M.I., Abdullaeva A.M. Sanitary assessment of poultry meat when using protein hydrolyzates in the diet

Poultry products are in high demand due to their high nutritional value, good taste, and affordability. The authors have studied and reported the statistics of poultry meat consumption in the country, the export potential of poultry products and the importance of poultry meat in human nutrition as a dietary product. The research shows how feed base helps to increase the competitiveness of poultry production – to improve qualitative characteristics of meat and reduce the accumulation of chemical contaminants. Attention is paid to the problem of infection of poultry products with pathogenic microorganisms that cause different severity of intoxication in humans, often with a fatal outcome. The authors' research is aimed at analyzing the risks to human health associated with the consumption of poultry meat sold in the retail network. Based on the data obtained proposals to improve production conditions and reduce the intensity of the contamination of poultry carcasses and meat products with disease pathogens were developed. Key words: food poisoning, hydrolyzates, nutritional and biological value of meat, pathogenic microorganisms, poultry meat, protein, safety indicators, symptoms.



56 Usadov T.R., Koltsov A.Yu., Sukher M.M., Morgunov Yu.P., Yanzhieva D.V., Salnikov N.I.  Test-system for detection of lumpy skin disease virus genome by Real-Time PCR

This article presents data on the development of a test-system for detection of lumpy skin disease (LSD) virus genome by Real-Time PCR. This technique is based on the amplification of a fragment (113 b.p.) of LSDV 129 gene using the original oligonucleotide primers and fluorescent-labeled hybridization probe. Analytical specificity of the developed test system was evaluated by testing the strains of LSD virus, sheep pox and goat pox viruses, contagious ecthyma virus, as well as biological samples from clinically healthy animals and intact cell cultures. The analytical sensitivity of the developed test-system was determined using tenfold serial dilutions cultural virus contained material, was 1,0±0,15 lg TCID50/ml. To create a positive control of amplification, the fragment of LSDV 129 gene synthesized during Real-Time PCR was cloned as part of the plasmid vector pGEM-T Easy in the E. coli bacterial cells. It was established that the range of plasmid DNA concentration, at which the amplification linearity is observed, is from 3,0х109 до 3,0 DNA copies/μl. Key-words: lumpy skin disease, virus, test-system, Real-Time PCR.


62 Suhinin A.A., Makavchick S.A., Yacentuk S.P.,  Zuev Yu.V.,  Shevcova L.I., Prasolova O.V. Validation of the developed technique of the detection in PCR the causative agent of virus diarrhea of cattle

Efficiency and detection limit (LOD) of the method were calculated, sensitivity and specificity of oligonucleotides were analyzed. To ensure the reproducibility of the study, two operators were conducted independently using different equipment of the same brand at different times. Positive control samples were developed. Proven a direct correlation of the threshold cycle Ct from the logarithm of the concentration of the DNA matrix, which is in the required range of measurements (R2≥0,98), which is also confirmed by the obtained values of the slope of the calibration curve (slope= –3,4; –3,4 and is –3,2). As a result of research it is shown that the analytical sensitivity of PCR is high (90, 70 and 30 copies of the target DNA, respectively), LOD is not more than 0,1 %, which meets the criteria. Specificity of oligonucleotides was checked on the panel of positive and negative samples. Within the working range of the method of false-positive and false-negative results was not revealed, indicating a high accuracy of the reaction. Key words: validation, viral diarrhea virus, the efficiency of reaction, limit of detection (LOD) techniques, the sensitivity and specificity of the work of oligonucleotides.


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CONTENTS №8-2019