CONTENTS №5-2016

3 Fedorov Yu.N., Klukina V.I., Bogomolova O.A., Romanenko M.N. Colostral immunity and immunoprophylaxis of newborn calves diseases Newborn calves posseses no resistance  against of the infectious agents present on the farm because of  their neonatal agammaglobulinemic status. This review discusses mechanisms  and factors of  passive immunity and describes  a study elated to failure its  transfer. The article presents  strategy and principles immunoprophylaxis  of newborn calves diseases.  Key words: calves, colostrum, immune system, immunity, immunodeficiencies, immunoprophylaxis.

PRACTIC: EXPERIMENT, PROBLEMS, PERSPECTIVES

8 Kontsevaya N.N., Soboleva G.L., Nepoklonova I.V., Aliper T.I. Vaccines KOMBOVAC 2 + L and KOMBOVAC 4+L  for creating colostral  immunity in young cattle

New vaccines against bovine infection diseases were development and described. Efficacy of the combined inactivated vaccines against infectious bovine rhinotracheitis, viral diarrhea, rotavirus, coronavirus and leptospirosis has been shown. Key words: viral diarrhea, infectious bovine rhinotracheitis, rotavirus, coronavirus, leptospirosis, cattle, antibodies, colostral immunity, vaccines.

13 Sarkisov К.А., Dmitrieva I.V. About the reasons of the claims on the vaccines against animal ringworm

The authors of paper analysis the reasons of claims for vaccines against animal ringworm. Recommendations are given for the correct use of these biopreparates. Key words:  dermatomycosis, vaccine, claims. 

INFECTIOUS  DISEASES  

15 Nikolaenko V.P., Karshin S.P., Kitz E.A., Binatova V.V., Lopteva M.S. Prevention and treatment of bovine necrobacteriosis 

There was developed a new antiseptic Brokarsept for prevention and treatment bovine necrobacillosis, optimized the scheme of its application and evaluated the cost-effectiveness of treatments animals. Key words: antiseptic, treatment,  necrobacillosis, prevention, epizootic process.

18 Puzankova O.S.,  Varensova A.A., Zhukov I.J., Pershin A.S., Remyga S.G., Gavrilova V.L.,  Elsukova A.A., Igolkin A.S., Gruzdev K.N., Vlasova N.N.   Reproduction of isolate Antonovo 07/14 ASFV in vivo and in vitro

The pathogenic isolate Antonovo 07/14 (A07) of the African swine fever (ASF) virus and the nature of its reproduction in bone marrow cell cultures that had infection and intact young pigs (YP) were studied. To study the biological properties of IZ, 24 ASF-virus seronegative 2 – 3 month-old pigs were selected, they were divided into 5 groups. The YP of group 1 were inoculated intramuscularly at the dose of 5 HadU/ cm3, the YP of group 2 – by the same method at the dose of 50 HadU/cm3, the YP of group 3 - by the same method at the dose of 5000 HadU/cm3, the YP of group 4 – intranasally at the dose of 50 HadU/cm3, intact YP of group 5 were kept together with infected animals. The YP of group 3 died on the 8 – 9-th day after inoculation, the YP of groups 1 and 2 – on the 9 – 10-th day, the pigs of groups 4 and 5 – on the 12 – 13-th day allowing thereby making a conclusion about homogeneity of the isolate A07 and confirming that the duration of the incubation period and death speed of animals directly depend on the infection dose. On the 28-th day survived YP N 995 (YP995) and 6 intact YP were intramuscularly inoculated with the IZ Gribovo 07/14 (G07) at the dose of 5000 HadU/ 2 cm3, after which it remained healthy, and in the intact YP distinctive clinical signs and the death on the 8-th day after infection were observed. The IZ genome was detected in blood of YP995 from the 4-th till 24-th day, in nasal lavages – on the 12 – 16-th day, in feces – on the 16-th day after its infection with IZ A07. On the 102-nd day of observation in YP995 the IZ ASF virus DNA in high concentration was detected in samples of kidneys, heart, mesenteric and mediastinal lymph nodes, ileocecal valve. The titer of antibodies to IZ ASF increased after 24 days to 1:100 and grew to the level of 1:8000 and 1:3200 after 59 and 102 days, respectively after infecting with IZ A07 and after 31 and 74 days – with IZ G07. When infecting the bone marrow cell culture with YP995 hemadsorption was identified after 36 – 48 h, and the healthy YP – after 24 h, in 5 days after inoculation of the isolate Odintsovo 02/14 the titer was 7,05±0,211 lg HadU50/0,15 cm3 and 5,83±0,13 lg HadU50/0,15 cm3, respectively. It has been concluded that the IZ A07 has virulent properties and propagates in the bone marrow cell culture of intact and convalescent YP. Key words: ASF virus, ELISA, bone marrow cell culture, PCR.  

24 Gulyukin M.I.,  Morugin A.K., Timakov A.V. The Influence of  seasons and management conditions on sheep morbidity with slow infection

The importance of fertility seasons ewes and the condition of immune reactivity of their organisms on the level of slow infections morbidity with nervous syndrome has been discovered. Positiveness of using range rearing of replacement ewes in their resistance to scrapie has been stated. Key words: slow infections, scrapie, Romanov breed of sheep, morbidity, fertility seasons.  

INVASIVE DISEASES

29 Arisov M.V., Indyuhova E.N., Arisova G.B., Stepanova I.A.  Chistotela zoospray gainst ectoparasites of rabbits

It was shown the therapeutic efficacy of Chistotel zoospray (Ecoprom, Russia) for rabbits with psoroptosis, sarcoptosis,  sifunkulatosis. Key words: rabbit, permethrin,  psoroptosis, sarcoptosis, sifunkulatosis, spray,  ectoparasites, therapeutic efficacy.

OBSTTETRICS, GYNECOLOGY

32 Gamayunov V.M., Amirov A.X.  The therapeutic efficacy of Vaccamastum for mastitis in lactating cows

The therapeutic efficacy of a new domestic anti-mastitis drug  Vaccamastum has been tested and the lactating cows of a big dairy farm against the efficacy of the Gamaret.

The incidence of serrous-catarrhal mastitis clinical mastitis was 4,4%, total – 14,7 %, hidden  – 10,3 %. The milk of the sick cows contained Escherichia coli, Streptococcus agalactia, S. uberus. Vaccamastum administered for the first time has manifested high therapeutic effectiveness: 84,5% of the experimental cows recovered 3 days, while only – 60,0 % in the control group. Key words: mastitis, therapeutic, Vaccamastum, efficacy.

34  Aliev A.Y. Mastitis in ewes

The article represents the material on the mastitis spread in ewes, its etiology, diagnostic techniques, therapy and prophylaxis. Key words: ewes, mastitis, spread, diagnosis, microflora of mammary gland secreta, treatment, prophylaxis.

ZOOHYGIENA, SANITATION, ECOLOGY

39 Tashbulatov A.A., Safiullin R.T., Gavrilova T.V. Complex cleaning, disinvasion of the equipment and rooms in broiler poultry farming

Complex sanitary hygiene program, disinfection and maintenance desinvasion of young broiler flocks and broiler breeders, at the floor maintenance, including the use of modern, efficient detergents, such as Bio Fome S, as well as disinfectants with high dezinfective features – Phenosteril. The article covers issues of complex preparation of facilities during a sanitary break for repair and parent stock broiler flocks, in order to maximize the free effect of birds from parasitic agents (nematodes and coccidiosis). Key words: comprehensive training facilities, repair and parent stock, Bio FOM S Phenosteril, poultry house, personnel, disinfection and disinfestation.

42 Saleeva I.P., Shol V.G., Ivanov A.V., Zotov A.A. Modern drugs for disinfection eggs prior to incubation

The review describes the drugs used for disinfection treatment of eggs before incubation, the results of studies of domestic and foreign scientists of study their impact on the reduction of microbial contamination, incubation efficiency & and quality of hatched chicks. Key words: disinfection, pre-incubation treatment, incubation, microbial contamination, poultry, egg.

NONINFECTIOUS DISEASES

47 Kochish I.I., Shukanov R.A. Development of the boar’s metabolism in local biogeochemical conditions of Chuvashia

Enrichment of ration by biogenic nutrient Kombiolaks and even more by natural zeolite trepel accompanied by a corrective effect on the metabolism of proteins, lipids, carbohydrates and minerals ofhogs in the south-east of Chuvashia. Key words: biogenic nutrients, blood, metabolism, pig, zeolite trepel.

50 Gertman A.M., Samsonova T.S., Rulikova E.M. Treatment of cows with hepatosis in thetechnogenic province of South Ural

Hepatosis of cows is widespread in the technogenic provinces and causes significant damage. Complex use of mineral sorbents, selenium drugs and symptomatic therapy gives a good therapeutic effect for treatment this disease. Key words: antioxidants, biogeochemical province, hepatosis, complex therapy, lipid peroxidation, selenium, sorbents, heavy metals, enterosorbent.

LABORATORY PRACTICE

54  Salnikov N.I., Strizhakova O.M., Suher  М.М., Tsybanov S.Zh., Kolbasov D.V. The test-system for detection of Swinepox virus DNA by Real-Time PCR

In the given article the stages of development of the test-system for detection of  Swinepox virus DNA by Real-Time are stated. The results of the test specificity and sensitivity of the test-system are also presented. Key words:  Swinepox, virus, Real-Time PCR, swine.

57 Plotnikova E.M., Ivanov A.V., Nizamov R.N., Konyukhov G.V., Gurianov N.I., Churina Z.G. The influence of γ-sterilization nutritient mediums by γ-radiation on cell cultures growth

The studies showed heat nutrient media irradiation with gamma rays at dosage of 2.104 Gr has a decontamination effect in the media microflora. The irradiation promotes cell the cultivated cells growth increasing cellular concentration for 1,96 times and proliferation  index-for 1,21 times than  of the cell grown in media decontaminated using the conventional 1 med hob, i.e. antoclaving. Key words: cell cultures, γ-rays, irradiation, sterilization.

VETERINARY-SANITARY CONTROL

59 Podzorova Yu.A., Kozak S.S., Seregin I.G. Veterinary and sanitary assessment slaughter products of hens with yolk peritonitis

There are show veterinary and sanitary assessment products of slaughter hens with yolk peritonitis. The results of these studies are recommended to supplement the section 4 of "Rules veterinary inspection of slaughter animals and veterinary-sanitary examination of meat and meat products". Key words: veterinary and sanitary examination, yolk peritonitis, laying hens.

61  FROM VETERINARY VEDICINE HISTORY